Effects of Presence of an Egg and Calcium Deposition in the Shell Gland on Levels of Messenger Ribonucleic Acid of CaBP-D28K and of Vitamin D3 Receptor in the Shell Gland of the Laying Hen

Teruko Ieda, Noboru Saito, Tamao Ono, Kiyoshi Shimada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Levels of calbindin-D28K (CaBP D28K) mRNA and vitamin D3, 1α, 25(OH)2D3, receptor (VDR) mRNA in the intestine and shell gland were measured in the laying hen during the ovulatory cycle by Northern and slot blot analyses. In addition, effects of presence of an egg and calcium deposition in the shell gland on the levels of both mRNAs were studied either by delay in oviposition (retaining the egg in the shell gland beyond the expected time of oviposition) or premature oviposition (emptying the shell gland and arrest of calcium deposition). During the ovulatory cycle mRNA levels of CaBP-D28K and of VDR remained relatively constant in the intestine. In contrast, both mRNA levels of the shell gland were low when there was an egg in the infundibulum, magnum, and isthmus, but significantly increased when there was an egg in the shell gland during shell formation. The im injection of indomethacin 3 hr before expected ovulation delayed oviposition but increased neither shell thickness nor mRNA levels of CaBP-D28K and of VDR. On the other hand, premature oviposition reduced mRNA levels of both CaBP-D28K and VDR in the shell gland. These results suggest that the presence of an egg and calcium deposition in the shell gland may be a stimulatory factor for synthesis and accumulation of CaBP-D28K mRNA and VDR mRNA in association with calcification.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume99
Issue number2
DOIs
Publication statusPublished - Aug 1995
Externally publishedYes

Fingerprint

shell gland
Calcitriol Receptors
cholecalciferol
laying hens
Ovum
RNA
Oviposition
Calcium
calcium
Messenger RNA
receptors
oviposition
Egg Shell
intestines
Intestines
calbindin
Calbindin 1
indomethacin
calcification
Cholecalciferol

ASJC Scopus subject areas

  • Endocrinology

Cite this

@article{17e367714c90419faf30a5af629798ef,
title = "Effects of Presence of an Egg and Calcium Deposition in the Shell Gland on Levels of Messenger Ribonucleic Acid of CaBP-D28K and of Vitamin D3 Receptor in the Shell Gland of the Laying Hen",
abstract = "Levels of calbindin-D28K (CaBP D28K) mRNA and vitamin D3, 1α, 25(OH)2D3, receptor (VDR) mRNA in the intestine and shell gland were measured in the laying hen during the ovulatory cycle by Northern and slot blot analyses. In addition, effects of presence of an egg and calcium deposition in the shell gland on the levels of both mRNAs were studied either by delay in oviposition (retaining the egg in the shell gland beyond the expected time of oviposition) or premature oviposition (emptying the shell gland and arrest of calcium deposition). During the ovulatory cycle mRNA levels of CaBP-D28K and of VDR remained relatively constant in the intestine. In contrast, both mRNA levels of the shell gland were low when there was an egg in the infundibulum, magnum, and isthmus, but significantly increased when there was an egg in the shell gland during shell formation. The im injection of indomethacin 3 hr before expected ovulation delayed oviposition but increased neither shell thickness nor mRNA levels of CaBP-D28K and of VDR. On the other hand, premature oviposition reduced mRNA levels of both CaBP-D28K and VDR in the shell gland. These results suggest that the presence of an egg and calcium deposition in the shell gland may be a stimulatory factor for synthesis and accumulation of CaBP-D28K mRNA and VDR mRNA in association with calcification.",
author = "Teruko Ieda and Noboru Saito and Tamao Ono and Kiyoshi Shimada",
year = "1995",
month = "8",
doi = "10.1006/gcen.1995.1095",
language = "English",
volume = "99",
pages = "145--151",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Effects of Presence of an Egg and Calcium Deposition in the Shell Gland on Levels of Messenger Ribonucleic Acid of CaBP-D28K and of Vitamin D3 Receptor in the Shell Gland of the Laying Hen

AU - Ieda, Teruko

AU - Saito, Noboru

AU - Ono, Tamao

AU - Shimada, Kiyoshi

PY - 1995/8

Y1 - 1995/8

N2 - Levels of calbindin-D28K (CaBP D28K) mRNA and vitamin D3, 1α, 25(OH)2D3, receptor (VDR) mRNA in the intestine and shell gland were measured in the laying hen during the ovulatory cycle by Northern and slot blot analyses. In addition, effects of presence of an egg and calcium deposition in the shell gland on the levels of both mRNAs were studied either by delay in oviposition (retaining the egg in the shell gland beyond the expected time of oviposition) or premature oviposition (emptying the shell gland and arrest of calcium deposition). During the ovulatory cycle mRNA levels of CaBP-D28K and of VDR remained relatively constant in the intestine. In contrast, both mRNA levels of the shell gland were low when there was an egg in the infundibulum, magnum, and isthmus, but significantly increased when there was an egg in the shell gland during shell formation. The im injection of indomethacin 3 hr before expected ovulation delayed oviposition but increased neither shell thickness nor mRNA levels of CaBP-D28K and of VDR. On the other hand, premature oviposition reduced mRNA levels of both CaBP-D28K and VDR in the shell gland. These results suggest that the presence of an egg and calcium deposition in the shell gland may be a stimulatory factor for synthesis and accumulation of CaBP-D28K mRNA and VDR mRNA in association with calcification.

AB - Levels of calbindin-D28K (CaBP D28K) mRNA and vitamin D3, 1α, 25(OH)2D3, receptor (VDR) mRNA in the intestine and shell gland were measured in the laying hen during the ovulatory cycle by Northern and slot blot analyses. In addition, effects of presence of an egg and calcium deposition in the shell gland on the levels of both mRNAs were studied either by delay in oviposition (retaining the egg in the shell gland beyond the expected time of oviposition) or premature oviposition (emptying the shell gland and arrest of calcium deposition). During the ovulatory cycle mRNA levels of CaBP-D28K and of VDR remained relatively constant in the intestine. In contrast, both mRNA levels of the shell gland were low when there was an egg in the infundibulum, magnum, and isthmus, but significantly increased when there was an egg in the shell gland during shell formation. The im injection of indomethacin 3 hr before expected ovulation delayed oviposition but increased neither shell thickness nor mRNA levels of CaBP-D28K and of VDR. On the other hand, premature oviposition reduced mRNA levels of both CaBP-D28K and VDR in the shell gland. These results suggest that the presence of an egg and calcium deposition in the shell gland may be a stimulatory factor for synthesis and accumulation of CaBP-D28K mRNA and VDR mRNA in association with calcification.

UR - http://www.scopus.com/inward/record.url?scp=0029147613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029147613&partnerID=8YFLogxK

U2 - 10.1006/gcen.1995.1095

DO - 10.1006/gcen.1995.1095

M3 - Article

C2 - 8536923

AN - SCOPUS:0029147613

VL - 99

SP - 145

EP - 151

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -