Abstract
Temporal changes in the acquisition of nitrogen (N) are recorded in tree-rings together with unique N isotopic values. Some debate continues regarding the importance of wood pre-treatment in isotope analysis and, thus, this study focuses on the removal of labile components to determine the intrinsic nature of N in tree-rings. The total concentration and stable isotopic value of N in annual tree-rings were determined for two cores from Japanese black pine (Pinus thunbergii) from areas colonized by black cormorant (Phalacrocorax carbo). One core sample was also collected from a control site, without cormorants. Sharp increases in tree-ring δ 15N values associated with migration of the cormorant population indicate positive incorporation of N from soils, whereas a less pronounced trend was observed for ring samples for periods without or substantially less migration, and for those obtained from the control site. All labile N components were removed by repeated extraction with toluene/ethanol (1:1) solution. Radial translocation of labile N is limited in tree-rings from Japanese black pine, providing intrinsic records on N acquisition. The difference in N isotopic values (up to 7.0°) following pre-treatment was statistically significant for trees affected by the avian colony, whereas the pre-treatment of the control samples did not influence N values. The implication is that in agreement with previous studies pre-treatment is not necessary when trees are exposed to natural N concentrations in the soil but the removal of enriched δ 15N labile components is necessary when woody plants are exposed to unusually high inputs of N into soils. However, the temporal trend in tree-ring δ 15N series of the avian N affected trees did not change. Thus, if the priority is not the value but the trend then pre-treatment is not necessary.
| Original language | English |
|---|---|
| Pages (from-to) | 3298-3302 |
| Number of pages | 5 |
| Journal | Rapid Communications in Mass Spectrometry |
| Volume | 25 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - Nov 15 2011 |
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ASJC Scopus subject areas
- Spectroscopy
- Analytical Chemistry
- Organic Chemistry
Cite this
Effects of pre-treatment on the nitrogen isotope composition of Japanese black pine (Pinus thunbergii) tree-rings as affected by high N input. / Larry Lopez Caceres, M.; Mizota, Chitoshi; Yamanaka, Toshiro; Nobori, Yoshihiro.
In: Rapid Communications in Mass Spectrometry, Vol. 25, No. 21, 15.11.2011, p. 3298-3302.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of pre-treatment on the nitrogen isotope composition of Japanese black pine (Pinus thunbergii) tree-rings as affected by high N input
AU - Larry Lopez Caceres, M.
AU - Mizota, Chitoshi
AU - Yamanaka, Toshiro
AU - Nobori, Yoshihiro
PY - 2011/11/15
Y1 - 2011/11/15
N2 - Temporal changes in the acquisition of nitrogen (N) are recorded in tree-rings together with unique N isotopic values. Some debate continues regarding the importance of wood pre-treatment in isotope analysis and, thus, this study focuses on the removal of labile components to determine the intrinsic nature of N in tree-rings. The total concentration and stable isotopic value of N in annual tree-rings were determined for two cores from Japanese black pine (Pinus thunbergii) from areas colonized by black cormorant (Phalacrocorax carbo). One core sample was also collected from a control site, without cormorants. Sharp increases in tree-ring δ 15N values associated with migration of the cormorant population indicate positive incorporation of N from soils, whereas a less pronounced trend was observed for ring samples for periods without or substantially less migration, and for those obtained from the control site. All labile N components were removed by repeated extraction with toluene/ethanol (1:1) solution. Radial translocation of labile N is limited in tree-rings from Japanese black pine, providing intrinsic records on N acquisition. The difference in N isotopic values (up to 7.0°) following pre-treatment was statistically significant for trees affected by the avian colony, whereas the pre-treatment of the control samples did not influence N values. The implication is that in agreement with previous studies pre-treatment is not necessary when trees are exposed to natural N concentrations in the soil but the removal of enriched δ 15N labile components is necessary when woody plants are exposed to unusually high inputs of N into soils. However, the temporal trend in tree-ring δ 15N series of the avian N affected trees did not change. Thus, if the priority is not the value but the trend then pre-treatment is not necessary.
AB - Temporal changes in the acquisition of nitrogen (N) are recorded in tree-rings together with unique N isotopic values. Some debate continues regarding the importance of wood pre-treatment in isotope analysis and, thus, this study focuses on the removal of labile components to determine the intrinsic nature of N in tree-rings. The total concentration and stable isotopic value of N in annual tree-rings were determined for two cores from Japanese black pine (Pinus thunbergii) from areas colonized by black cormorant (Phalacrocorax carbo). One core sample was also collected from a control site, without cormorants. Sharp increases in tree-ring δ 15N values associated with migration of the cormorant population indicate positive incorporation of N from soils, whereas a less pronounced trend was observed for ring samples for periods without or substantially less migration, and for those obtained from the control site. All labile N components were removed by repeated extraction with toluene/ethanol (1:1) solution. Radial translocation of labile N is limited in tree-rings from Japanese black pine, providing intrinsic records on N acquisition. The difference in N isotopic values (up to 7.0°) following pre-treatment was statistically significant for trees affected by the avian colony, whereas the pre-treatment of the control samples did not influence N values. The implication is that in agreement with previous studies pre-treatment is not necessary when trees are exposed to natural N concentrations in the soil but the removal of enriched δ 15N labile components is necessary when woody plants are exposed to unusually high inputs of N into soils. However, the temporal trend in tree-ring δ 15N series of the avian N affected trees did not change. Thus, if the priority is not the value but the trend then pre-treatment is not necessary.
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U2 - 10.1002/rcm.5227
DO - 10.1002/rcm.5227
M3 - Article
C2 - 22006393
AN - SCOPUS:80054986450
VL - 25
SP - 3298
EP - 3302
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
SN - 0951-4198
IS - 21
ER -