Effects of PKC inhibitors on suppression of thermotolerance development in tsAF8 cells

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Abstract

Effects of protein kinase C (PKC) inhibitors (H7, staurosporine, calphostin C) on thermotolerance development were investigated in temperature sensitive tsAF8 cells derived from Syrian hamster BHK21 cells. Cells were pre-heated at 45°C for 20 min, incubated at 34°C with PKC inhibitors for varying lengths of time, i.e. 1.25-10.0h, and then heated at 45°C for 30 min. Increasing survival fractions after the second heat treatment was inhibited by the treatment with H7 (40-160 μM), with staurosporine (0.05-1.0 μM), and with calphostin C (0.8, 1.2 μM) in a concentration dependent manner. When the concentrations of these PKC inhibitors were low, the restraint of increasing survival fractions was temporary, since survival fractions increased 3-7.5h after pre-heating. However, the survival fractions were almost constant by the treatment with 160 μM H7 and 1.0 μM staurosporine. Induction of HSP72 after heat stress was investigated in tsAF8 and BHK21 cells. Cells were heated at 45°C for 20 min and incubated at 34 or 39.7°C (tsAF8), at 37°C (BHK21). Intensity of intracellular fluoresence from HSP72 was measured by flow cytometry. HSP72 was induced in BHK21 cells, but there was no definite induction of HSP72 in tsAF8 cells at either 39.7 or 34°C. These results suggest that PKC is related with the thermotolerance development in tsAF8 cells; however, HSP72 is not involved in the thermotolerance development in tsAF8 cells.

Original languageEnglish
Pages (from-to)106-113
Number of pages8
JournalInternational Journal of Hyperthermia
Volume17
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Protein C Inhibitor
Protein Kinase Inhibitors
Protein Kinase C
Staurosporine
Hot Temperature
Thermotolerance
Mesocricetus
Heating
Flow Cytometry
Temperature

Keywords

  • Hyperthermia
  • PKC inhibitor
  • Thermotolerance
  • tsAF8

ASJC Scopus subject areas

  • Cancer Research
  • Radiological and Ultrasound Technology

Cite this

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title = "Effects of PKC inhibitors on suppression of thermotolerance development in tsAF8 cells",
abstract = "Effects of protein kinase C (PKC) inhibitors (H7, staurosporine, calphostin C) on thermotolerance development were investigated in temperature sensitive tsAF8 cells derived from Syrian hamster BHK21 cells. Cells were pre-heated at 45°C for 20 min, incubated at 34°C with PKC inhibitors for varying lengths of time, i.e. 1.25-10.0h, and then heated at 45°C for 30 min. Increasing survival fractions after the second heat treatment was inhibited by the treatment with H7 (40-160 μM), with staurosporine (0.05-1.0 μM), and with calphostin C (0.8, 1.2 μM) in a concentration dependent manner. When the concentrations of these PKC inhibitors were low, the restraint of increasing survival fractions was temporary, since survival fractions increased 3-7.5h after pre-heating. However, the survival fractions were almost constant by the treatment with 160 μM H7 and 1.0 μM staurosporine. Induction of HSP72 after heat stress was investigated in tsAF8 and BHK21 cells. Cells were heated at 45°C for 20 min and incubated at 34 or 39.7°C (tsAF8), at 37°C (BHK21). Intensity of intracellular fluoresence from HSP72 was measured by flow cytometry. HSP72 was induced in BHK21 cells, but there was no definite induction of HSP72 in tsAF8 cells at either 39.7 or 34°C. These results suggest that PKC is related with the thermotolerance development in tsAF8 cells; however, HSP72 is not involved in the thermotolerance development in tsAF8 cells.",
keywords = "Hyperthermia, PKC inhibitor, Thermotolerance, tsAF8",
author = "Koichi Shibuya and S. Kawasaki and Masahiro Kuroda and Jun-Ichi Asaumi and Susumu Kanazawa and Y. Hiraki",
year = "2001",
doi = "10.1080/02656730010010667",
language = "English",
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journal = "International Journal of Hyperthermia",
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TY - JOUR

T1 - Effects of PKC inhibitors on suppression of thermotolerance development in tsAF8 cells

AU - Shibuya, Koichi

AU - Kawasaki, S.

AU - Kuroda, Masahiro

AU - Asaumi, Jun-Ichi

AU - Kanazawa, Susumu

AU - Hiraki, Y.

PY - 2001

Y1 - 2001

N2 - Effects of protein kinase C (PKC) inhibitors (H7, staurosporine, calphostin C) on thermotolerance development were investigated in temperature sensitive tsAF8 cells derived from Syrian hamster BHK21 cells. Cells were pre-heated at 45°C for 20 min, incubated at 34°C with PKC inhibitors for varying lengths of time, i.e. 1.25-10.0h, and then heated at 45°C for 30 min. Increasing survival fractions after the second heat treatment was inhibited by the treatment with H7 (40-160 μM), with staurosporine (0.05-1.0 μM), and with calphostin C (0.8, 1.2 μM) in a concentration dependent manner. When the concentrations of these PKC inhibitors were low, the restraint of increasing survival fractions was temporary, since survival fractions increased 3-7.5h after pre-heating. However, the survival fractions were almost constant by the treatment with 160 μM H7 and 1.0 μM staurosporine. Induction of HSP72 after heat stress was investigated in tsAF8 and BHK21 cells. Cells were heated at 45°C for 20 min and incubated at 34 or 39.7°C (tsAF8), at 37°C (BHK21). Intensity of intracellular fluoresence from HSP72 was measured by flow cytometry. HSP72 was induced in BHK21 cells, but there was no definite induction of HSP72 in tsAF8 cells at either 39.7 or 34°C. These results suggest that PKC is related with the thermotolerance development in tsAF8 cells; however, HSP72 is not involved in the thermotolerance development in tsAF8 cells.

AB - Effects of protein kinase C (PKC) inhibitors (H7, staurosporine, calphostin C) on thermotolerance development were investigated in temperature sensitive tsAF8 cells derived from Syrian hamster BHK21 cells. Cells were pre-heated at 45°C for 20 min, incubated at 34°C with PKC inhibitors for varying lengths of time, i.e. 1.25-10.0h, and then heated at 45°C for 30 min. Increasing survival fractions after the second heat treatment was inhibited by the treatment with H7 (40-160 μM), with staurosporine (0.05-1.0 μM), and with calphostin C (0.8, 1.2 μM) in a concentration dependent manner. When the concentrations of these PKC inhibitors were low, the restraint of increasing survival fractions was temporary, since survival fractions increased 3-7.5h after pre-heating. However, the survival fractions were almost constant by the treatment with 160 μM H7 and 1.0 μM staurosporine. Induction of HSP72 after heat stress was investigated in tsAF8 and BHK21 cells. Cells were heated at 45°C for 20 min and incubated at 34 or 39.7°C (tsAF8), at 37°C (BHK21). Intensity of intracellular fluoresence from HSP72 was measured by flow cytometry. HSP72 was induced in BHK21 cells, but there was no definite induction of HSP72 in tsAF8 cells at either 39.7 or 34°C. These results suggest that PKC is related with the thermotolerance development in tsAF8 cells; however, HSP72 is not involved in the thermotolerance development in tsAF8 cells.

KW - Hyperthermia

KW - PKC inhibitor

KW - Thermotolerance

KW - tsAF8

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U2 - 10.1080/02656730010010667

DO - 10.1080/02656730010010667

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VL - 17

SP - 106

EP - 113

JO - International Journal of Hyperthermia

JF - International Journal of Hyperthermia

SN - 0265-6736

IS - 2

ER -