Effects of periodontitis on aortic insulin resistance in an obese rat model

Daisuke Ekuni, Takaaki Tomofuji, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Tetsuji Azuma, Naofumi Tamaki, Toshihiro Sanbe, Yasumasa Endo, Tatsuo Yamamoto, Takashi Nishida, Manabu Morita

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The combination of obesity and its associated risk factors, such as insulin resistance and inflammation, results in the development of atherosclerosis. However, the effects of periodontitis on atherosclerosis in an obese body remain unclear. The aim of the study was to investigate the effects of ligature-induced periodontitis in Zucker fatty rats on initiation of atherosclerosis by evaluating aortic insulin resistance. Zucker fatty rats (n24) were divided into two groups. In the periodontitis group, periodontitis was ligature-induced for 4 weeks, whereas the control group was left unligated. After the 4-week experimental period, descending aorta was used for measuring the levels of lipid deposits, immunohistochemical analysis, and evaluation of gene expression. Levels of serum C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), and insulin were also measured. Rats in the periodontitis group had significantly enhanced lipid deposits in the aorta, but not in the control group. Expression of suppressor of cytokine signaling 3, vascular cell adhesion molecule 1, reactive oxygen species, nitrotyrosine, and endothelin-1 in the periodontitis group was more intense than that in the control group. Significantly decreased levels of phosphatidylinositol 3-kinase (Pi3k) catalytic Β-polypeptide (Pi3kcb), Pi3kp85, and insulin receptor substrate 1 and 2 were observed in the periodontitis group. Levels of serum CRP and TNF-α were significantly increased in the periodontitis group. Under insulin-stimulated conditions, aorta in the periodontitis group altered the Akt phosphorylation. Periodontitis in obesity induced the initial stage of atherosclerosis and disturbed aortic insulin signaling.

Original languageEnglish
Pages (from-to)348-359
Number of pages12
JournalLaboratory Investigation
Volume90
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Periodontitis
Insulin Resistance
Atherosclerosis
Zucker Rats
Insulin Receptor Substrate Proteins
Insulin
C-Reactive Protein
Control Groups
Ligation
Aorta
Blood Proteins
Tumor Necrosis Factor-alpha
Obesity
Phosphatidylinositol 3-Kinase
Lipids
Vascular Cell Adhesion Molecule-1
Endothelin-1
Thoracic Aorta
Reactive Oxygen Species
Phosphorylation

Keywords

  • Animal models
  • Atherosclerosis
  • Insulin resistance
  • Obesity
  • Periodontal disease

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Effects of periodontitis on aortic insulin resistance in an obese rat model. / Ekuni, Daisuke; Tomofuji, Takaaki; Irie, Koichiro; Kasuyama, Kenta; Umakoshi, Michihiro; Azuma, Tetsuji; Tamaki, Naofumi; Sanbe, Toshihiro; Endo, Yasumasa; Yamamoto, Tatsuo; Nishida, Takashi; Morita, Manabu.

In: Laboratory Investigation, Vol. 90, No. 3, 03.2010, p. 348-359.

Research output: Contribution to journalArticle

Ekuni, D, Tomofuji, T, Irie, K, Kasuyama, K, Umakoshi, M, Azuma, T, Tamaki, N, Sanbe, T, Endo, Y, Yamamoto, T, Nishida, T & Morita, M 2010, 'Effects of periodontitis on aortic insulin resistance in an obese rat model', Laboratory Investigation, vol. 90, no. 3, pp. 348-359. https://doi.org/10.1038/labinvest.2009.141
Ekuni, Daisuke ; Tomofuji, Takaaki ; Irie, Koichiro ; Kasuyama, Kenta ; Umakoshi, Michihiro ; Azuma, Tetsuji ; Tamaki, Naofumi ; Sanbe, Toshihiro ; Endo, Yasumasa ; Yamamoto, Tatsuo ; Nishida, Takashi ; Morita, Manabu. / Effects of periodontitis on aortic insulin resistance in an obese rat model. In: Laboratory Investigation. 2010 ; Vol. 90, No. 3. pp. 348-359.
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