Effects of Oxidative Stress and Inhibitors of the Pentose Phosphate Pathway on Sexually Dimorphic Production of IFN-τ by Bovine Blastocysts

Koji Kimura, L. D. Spate, M. P. Green, R. M. Roberts

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Bovine interferon-tau (IFN-τ), the anti-luteolytic factor secreted by conceptuses of pecoran ruminants, is a product of autosomal genes, yet in vitro produced (IVP) female expanded blastocysts (EB) secrete about twice as much IFN-τ as males. Two possible explanations have been tested here. One is that embryos of one sex are differentially susceptible to oxidative stress. The second is that female EB produce more IFN-τ because pentose-phosphate pathway (PPP) activity is elevated as a result of delayed X-chromosome inactivation. IVP bovine zygotes were cultured to the 8-cell stage and placed under conditions designed either to promote oxidative stress (±H 2O2; 20 vs. 5% O2), or to inhibit glucose 6-phosphate dehydrogenase (G6PDH) activity (addition of dehydroepiandrosterone, DHEA or 6-aminonicotinamide, 6-AN to the medium). At day 8, blastocysts were cultured individually for a further 48 hr to assess IFN-τ production, and embryo sex determined retrospectively. Blastocyst numbers were reduced (P <0.05) and their continued development impaired (P <0.05) in presence of H2O2 (200 μM) and 20% O2, but neither IFN-τ production nor sexually dimorphic expression of IFN-τ were affected. IFN-τ production was reduced, particularly in females (P <0.05), and sexual dimorphic differences in production were lost in the presence of both DHEA (100 μM) and 6-AN (1 μM). In the case of 6-AN, these effects were achieved without a significant decline in blastocyst developmental progression, quality, or cell number. The data suggest that the higher production of IFN-τ by female EB is an indirect outcome of the increased activity of the oxidative arm of the PPP pathway.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalMolecular Reproduction and Development
Volume68
Issue number1
DOIs
Publication statusPublished - May 2004
Externally publishedYes

Fingerprint

Pentose Phosphate Pathway
Blastocyst
Oxidative Stress
Dehydroepiandrosterone
6-Aminonicotinamide
Embryonic Structures
X Chromosome Inactivation
trophoblastin
Zygote
Glucosephosphate Dehydrogenase
Ruminants
Cell Count

Keywords

  • Bovine blastocyst
  • Embryo development
  • Interferon
  • Maternal recognition of pregnancy
  • Sexual dimorphism

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Effects of Oxidative Stress and Inhibitors of the Pentose Phosphate Pathway on Sexually Dimorphic Production of IFN-τ by Bovine Blastocysts. / Kimura, Koji; Spate, L. D.; Green, M. P.; Roberts, R. M.

In: Molecular Reproduction and Development, Vol. 68, No. 1, 05.2004, p. 88-95.

Research output: Contribution to journalArticle

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abstract = "Bovine interferon-tau (IFN-τ), the anti-luteolytic factor secreted by conceptuses of pecoran ruminants, is a product of autosomal genes, yet in vitro produced (IVP) female expanded blastocysts (EB) secrete about twice as much IFN-τ as males. Two possible explanations have been tested here. One is that embryos of one sex are differentially susceptible to oxidative stress. The second is that female EB produce more IFN-τ because pentose-phosphate pathway (PPP) activity is elevated as a result of delayed X-chromosome inactivation. IVP bovine zygotes were cultured to the 8-cell stage and placed under conditions designed either to promote oxidative stress (±H 2O2; 20 vs. 5{\%} O2), or to inhibit glucose 6-phosphate dehydrogenase (G6PDH) activity (addition of dehydroepiandrosterone, DHEA or 6-aminonicotinamide, 6-AN to the medium). At day 8, blastocysts were cultured individually for a further 48 hr to assess IFN-τ production, and embryo sex determined retrospectively. Blastocyst numbers were reduced (P <0.05) and their continued development impaired (P <0.05) in presence of H2O2 (200 μM) and 20{\%} O2, but neither IFN-τ production nor sexually dimorphic expression of IFN-τ were affected. IFN-τ production was reduced, particularly in females (P <0.05), and sexual dimorphic differences in production were lost in the presence of both DHEA (100 μM) and 6-AN (1 μM). In the case of 6-AN, these effects were achieved without a significant decline in blastocyst developmental progression, quality, or cell number. The data suggest that the higher production of IFN-τ by female EB is an indirect outcome of the increased activity of the oxidative arm of the PPP pathway.",
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