Effects of OPC-21268, a vasopressin V1-receptor antagonist, on expression of growth factors from glomeruli in spontaneously hypertensive rats

Fumio Otsuka, Toshio Ogura, Takayoshi Yamauchi, Tetsuya Oishi, Masami Hashimoto, Yukari Mimura, Hirofumi Makino

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To assess the chronic in vivo effects of OPC-21268, a vasopressin-V1 receptor antagonist, on renal injury, we investigated the mRNA expressions of platelet-derived growth factor (PDGF) B-chain, transforming growth factor (TGF)-β1 and proliferating cell nuclear antigen (PCNA) in the glomeruli of spontaneously hypertensive rats (SHR) treated with OPC-21268 for 3 weeks. SHR aged 10 weeks were given 2% NaCl in drinking water for 3 weeks. The OPC group was fed a 0.5% OPC-21268-containing diet for 3 weeks and the control group was given a normal diet. There were no significant changes in the time course of systolic blood pressure, heart rate, urine volume, or urinary sodium, protein and N-acetyl-β-glucosaminidase (NAG) excretion between the two groups. Serum electrolytes, protein and creatinine levels also did not differ between the groups. The mRNA expressions of PDGF B-chain, TGF-β1 and PCNA in the glomerulus were examined using reverse transcriptase-polymerase chain reaction (RT-PCR) methods. The mRNA expressions of PDGF B-chain and PCNA among these were significantly suppressed in the OPC group. No significant differences in renal histology including the organ weights were found between the two groups; however, the glomerular size tended to be enlarged in the OPC group. These findings suggest that chronic V1-receptor blockade directly inhibits the glomerular proliferative injury of salt-loaded SHR at the established hypertension stage.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalRegulatory Peptides
Volume72
Issue number2-3
DOIs
Publication statusPublished - Oct 31 1997

Fingerprint

OPC 21268
Proto-Oncogene Proteins c-sis
Vasopressin Receptors
Proliferating Cell Nuclear Antigen
Inbred SHR Rats
Vasopressins
Rats
Intercellular Signaling Peptides and Proteins
Transforming Growth Factors
Nutrition
Messenger RNA
Diet
Blood Pressure
Kidney
Hexosaminidases
Histology
Organ Size
Polymerase chain reaction
RNA-Directed DNA Polymerase
Blood pressure

Keywords

  • Glomerulus
  • Platelet-derived growth factor (PDGF) B-chain
  • Proliferating cell nuclear antigen (PCNA)
  • Spontaneously hypertensive rat (SHR)
  • V1-receptor antagonist
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Effects of OPC-21268, a vasopressin V1-receptor antagonist, on expression of growth factors from glomeruli in spontaneously hypertensive rats. / Otsuka, Fumio; Ogura, Toshio; Yamauchi, Takayoshi; Oishi, Tetsuya; Hashimoto, Masami; Mimura, Yukari; Makino, Hirofumi.

In: Regulatory Peptides, Vol. 72, No. 2-3, 31.10.1997, p. 87-95.

Research output: Contribution to journalArticle

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