Effects of OPC-21268, a vasopressin V1-receptor antagonist, on expression of growth factors from glomeruli in spontaneously hypertensive rats

Fumio Otsuka, Toshio Ogura, Takayoshi Yamauchi, Tetsuya Oishi, Masami Hashimoto, Yukari Mimura, Hirofumi Makino

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

To assess the chronic in vivo effects of OPC-21268, a vasopressin-V1 receptor antagonist, on renal injury, we investigated the mRNA expressions of platelet-derived growth factor (PDGF) B-chain, transforming growth factor (TGF)-β1 and proliferating cell nuclear antigen (PCNA) in the glomeruli of spontaneously hypertensive rats (SHR) treated with OPC-21268 for 3 weeks. SHR aged 10 weeks were given 2% NaCl in drinking water for 3 weeks. The OPC group was fed a 0.5% OPC-21268-containing diet for 3 weeks and the control group was given a normal diet. There were no significant changes in the time course of systolic blood pressure, heart rate, urine volume, or urinary sodium, protein and N-acetyl-β-glucosaminidase (NAG) excretion between the two groups. Serum electrolytes, protein and creatinine levels also did not differ between the groups. The mRNA expressions of PDGF B-chain, TGF-β1 and PCNA in the glomerulus were examined using reverse transcriptase-polymerase chain reaction (RT-PCR) methods. The mRNA expressions of PDGF B-chain and PCNA among these were significantly suppressed in the OPC group. No significant differences in renal histology including the organ weights were found between the two groups; however, the glomerular size tended to be enlarged in the OPC group. These findings suggest that chronic V1-receptor blockade directly inhibits the glomerular proliferative injury of salt-loaded SHR at the established hypertension stage.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalRegulatory Peptides
Volume72
Issue number2-3
DOIs
Publication statusPublished - Oct 31 1997

Keywords

  • Glomerulus
  • Platelet-derived growth factor (PDGF) B-chain
  • Proliferating cell nuclear antigen (PCNA)
  • Spontaneously hypertensive rat (SHR)
  • V1-receptor antagonist
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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