Effects of obesity on gingival oxidative stress in a rat model

Takaaki Tomofuji, Tatsuo Yamamoto, Naofumi Tamaki, Daisuke Ekuni, Tetsuji Azuma, Toshihiro Sanbe, Koichiro Irie, Kenta Kasuyama, Michihiro Umakoshi, Jun Murakami, Susumu Kokeguchi, Manabu Morita

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Studies indicate acorrelationbetweenobesity and periodontitis. Oxidative stress is involved in the progression of periodontitis. The purpose of this study was to investigate the effects of obesity on gingival oxidative stress in a rat periodontitis model. Methods: The obese Zucker rats (n = 14) and their lean lit-termates (n = 14) were each divided into two groups of seven rats. In one of each group, periodontitis was induced by ligature for 4 weeks, whereas the other group was left unligated. The level of 8-hydroxydeoxyguanosine and the ratio of reduced/oxidized glutathione were determined to examine gin-gival oxidative stress. The serum level of reactive oxygen metabolites and the gingival gene-expression pattern related to oxidative/metabolic stress, inflammation, and cell behavior were also evaluated. Results: The obese rats weighed more than the lean rats at 4 weeks. Compared to lean rats, obese rats had enhanced gin-gival 8-hydroxydeoxyguanosine levels and a decreased ratio of reduced/oxidized glutathione in the gingival tissue, with increasing serum reactive oxygen metabolites. However, there were no significant differences in the degree of alveolar bone loss between lean and obese rats, except for teeth with and without ligatures in both rats. In addition, the periodontal lesion in obese rats showed higher 8-hydroxydeoxyguanosine levels and polymorphonuclear leukocyte infiltration than the inflamed ones in lean rats, with downregulation of multiple cytochrome P450 gene expression. Conclusions: Obesity induced gingival oxidative stress with increasing serum reactive oxygen metabolites in rats. In the periodontal lesion, gene expressions related to a capacity for xenobiotic detoxification were downregulated in the obese.

Original languageEnglish
Pages (from-to)1324-1329
Number of pages6
JournalJournal of Periodontology
Volume80
Issue number8
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Oxidative Stress
Obesity
Periodontitis
Glutathione Disulfide
Oxygen
Gene Expression
Glutathione
Ligation
Down-Regulation
Serum
Alveolar Bone Loss
Zucker Rats
Physiological Stress
Xenobiotics
Cytochrome P-450 Enzyme System
Tooth
Neutrophils
Inflammation

Keywords

  • Obesity
  • Oxidative stress
  • Periodontal disese
  • Zucker rats

ASJC Scopus subject areas

  • Periodontics

Cite this

Effects of obesity on gingival oxidative stress in a rat model. / Tomofuji, Takaaki; Yamamoto, Tatsuo; Tamaki, Naofumi; Ekuni, Daisuke; Azuma, Tetsuji; Sanbe, Toshihiro; Irie, Koichiro; Kasuyama, Kenta; Umakoshi, Michihiro; Murakami, Jun; Kokeguchi, Susumu; Morita, Manabu.

In: Journal of Periodontology, Vol. 80, No. 8, 08.2009, p. 1324-1329.

Research output: Contribution to journalArticle

Tomofuji, T, Yamamoto, T, Tamaki, N, Ekuni, D, Azuma, T, Sanbe, T, Irie, K, Kasuyama, K, Umakoshi, M, Murakami, J, Kokeguchi, S & Morita, M 2009, 'Effects of obesity on gingival oxidative stress in a rat model', Journal of Periodontology, vol. 80, no. 8, pp. 1324-1329. https://doi.org/10.1902/jop.2009.080621
Tomofuji, Takaaki ; Yamamoto, Tatsuo ; Tamaki, Naofumi ; Ekuni, Daisuke ; Azuma, Tetsuji ; Sanbe, Toshihiro ; Irie, Koichiro ; Kasuyama, Kenta ; Umakoshi, Michihiro ; Murakami, Jun ; Kokeguchi, Susumu ; Morita, Manabu. / Effects of obesity on gingival oxidative stress in a rat model. In: Journal of Periodontology. 2009 ; Vol. 80, No. 8. pp. 1324-1329.
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abstract = "Background: Studies indicate acorrelationbetweenobesity and periodontitis. Oxidative stress is involved in the progression of periodontitis. The purpose of this study was to investigate the effects of obesity on gingival oxidative stress in a rat periodontitis model. Methods: The obese Zucker rats (n = 14) and their lean lit-termates (n = 14) were each divided into two groups of seven rats. In one of each group, periodontitis was induced by ligature for 4 weeks, whereas the other group was left unligated. The level of 8-hydroxydeoxyguanosine and the ratio of reduced/oxidized glutathione were determined to examine gin-gival oxidative stress. The serum level of reactive oxygen metabolites and the gingival gene-expression pattern related to oxidative/metabolic stress, inflammation, and cell behavior were also evaluated. Results: The obese rats weighed more than the lean rats at 4 weeks. Compared to lean rats, obese rats had enhanced gin-gival 8-hydroxydeoxyguanosine levels and a decreased ratio of reduced/oxidized glutathione in the gingival tissue, with increasing serum reactive oxygen metabolites. However, there were no significant differences in the degree of alveolar bone loss between lean and obese rats, except for teeth with and without ligatures in both rats. In addition, the periodontal lesion in obese rats showed higher 8-hydroxydeoxyguanosine levels and polymorphonuclear leukocyte infiltration than the inflamed ones in lean rats, with downregulation of multiple cytochrome P450 gene expression. Conclusions: Obesity induced gingival oxidative stress with increasing serum reactive oxygen metabolites in rats. In the periodontal lesion, gene expressions related to a capacity for xenobiotic detoxification were downregulated in the obese.",
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AU - Sanbe, Toshihiro

AU - Irie, Koichiro

AU - Kasuyama, Kenta

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KW - Periodontal disese

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