Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase

A randomized double-blind clinical trial

K. Takeuchi-Hatanaka, T. Yasuda, K. Naruishi, K. Katsuragi-Fuke, J. Inubushi, H. Ootsuki, H. Maeda, Shogo Takashiba

Research output: Contribution to journalArticle

Abstract

Background and Objective: The biochemical effects of an over-the-counter (OTC) medication were studied, which consists of a single-tuft brush containing cetylpyridinium chloride as a bactericidal agent, dipotassium glycyrrhizate as an anti-inflammatory drug and allantoin as a promoter of cell proliferation and wound healing, for delivery to hardly brushed sites. Material and Methods: This randomized controlled double-blind study was performed in 61 subjects with chronic periodontitis in supportive periodontal therapy phase (test group: n = 27; placebo group: n = 28; dropout: n = 6). The OTC medication was self-applied twice a day for 12 wk to two molars with probing pocket depths of 4-6 mm. Biochemical indicators were evaluated at baseline and 12 wk using the suspension array system for eight cytokines and chemokines (interleukin [IL]-1β, IL-1ra, IL-4, IL-6, IL-8, IL-10, monocyte chemoattractant protein-1 and tumor necrosis factor [TNF]-α) in gingival crevicular fluid. Results: The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other. Conclusion: This OTC medication is biochemically effective for steady chronic periodontitis in the supportive periodontal therapy phase.

Original languageEnglish
JournalJournal of Periodontal Research
DOIs
Publication statusAccepted/In press - 2015

Fingerprint

Gingival Crevicular Fluid
Ointments
Interleukin-1
Clinical Trials
Cytokines
Interleukin-6
Chronic Periodontitis
Tumor Necrosis Factor-alpha
Placebos
Interleukin-8
Allantoin
Cetylpyridinium
Therapeutics
Chemokine CCL2
Interleukins
Tuberculin
Chemokines
Double-Blind Method
Interleukin-4
Interleukin-10

Keywords

  • Inflammatory cytokine
  • Over-the-counter medication
  • Self-medication
  • Supportive periodontal therapy

ASJC Scopus subject areas

  • Periodontics

Cite this

@article{183a3ba8bf864fa2b4a244b08bc1f6c1,
title = "Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase: A randomized double-blind clinical trial",
abstract = "Background and Objective: The biochemical effects of an over-the-counter (OTC) medication were studied, which consists of a single-tuft brush containing cetylpyridinium chloride as a bactericidal agent, dipotassium glycyrrhizate as an anti-inflammatory drug and allantoin as a promoter of cell proliferation and wound healing, for delivery to hardly brushed sites. Material and Methods: This randomized controlled double-blind study was performed in 61 subjects with chronic periodontitis in supportive periodontal therapy phase (test group: n = 27; placebo group: n = 28; dropout: n = 6). The OTC medication was self-applied twice a day for 12 wk to two molars with probing pocket depths of 4-6 mm. Biochemical indicators were evaluated at baseline and 12 wk using the suspension array system for eight cytokines and chemokines (interleukin [IL]-1β, IL-1ra, IL-4, IL-6, IL-8, IL-10, monocyte chemoattractant protein-1 and tumor necrosis factor [TNF]-α) in gingival crevicular fluid. Results: The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other. Conclusion: This OTC medication is biochemically effective for steady chronic periodontitis in the supportive periodontal therapy phase.",
keywords = "Inflammatory cytokine, Over-the-counter medication, Self-medication, Supportive periodontal therapy",
author = "K. Takeuchi-Hatanaka and T. Yasuda and K. Naruishi and K. Katsuragi-Fuke and J. Inubushi and H. Ootsuki and H. Maeda and Shogo Takashiba",
year = "2015",
doi = "10.1111/jre.12311",
language = "English",
journal = "Journal of Periodontal Research",
issn = "0022-3484",
publisher = "Blackwell Munksgaard",

}

TY - JOUR

T1 - Effects of new over-the-counter periodontal ointment-containing applicator with single-tuft brush on cytokine levels in gingival crevicular fluid during supportive periodontal therapy phase

T2 - A randomized double-blind clinical trial

AU - Takeuchi-Hatanaka, K.

AU - Yasuda, T.

AU - Naruishi, K.

AU - Katsuragi-Fuke, K.

AU - Inubushi, J.

AU - Ootsuki, H.

AU - Maeda, H.

AU - Takashiba, Shogo

PY - 2015

Y1 - 2015

N2 - Background and Objective: The biochemical effects of an over-the-counter (OTC) medication were studied, which consists of a single-tuft brush containing cetylpyridinium chloride as a bactericidal agent, dipotassium glycyrrhizate as an anti-inflammatory drug and allantoin as a promoter of cell proliferation and wound healing, for delivery to hardly brushed sites. Material and Methods: This randomized controlled double-blind study was performed in 61 subjects with chronic periodontitis in supportive periodontal therapy phase (test group: n = 27; placebo group: n = 28; dropout: n = 6). The OTC medication was self-applied twice a day for 12 wk to two molars with probing pocket depths of 4-6 mm. Biochemical indicators were evaluated at baseline and 12 wk using the suspension array system for eight cytokines and chemokines (interleukin [IL]-1β, IL-1ra, IL-4, IL-6, IL-8, IL-10, monocyte chemoattractant protein-1 and tumor necrosis factor [TNF]-α) in gingival crevicular fluid. Results: The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other. Conclusion: This OTC medication is biochemically effective for steady chronic periodontitis in the supportive periodontal therapy phase.

AB - Background and Objective: The biochemical effects of an over-the-counter (OTC) medication were studied, which consists of a single-tuft brush containing cetylpyridinium chloride as a bactericidal agent, dipotassium glycyrrhizate as an anti-inflammatory drug and allantoin as a promoter of cell proliferation and wound healing, for delivery to hardly brushed sites. Material and Methods: This randomized controlled double-blind study was performed in 61 subjects with chronic periodontitis in supportive periodontal therapy phase (test group: n = 27; placebo group: n = 28; dropout: n = 6). The OTC medication was self-applied twice a day for 12 wk to two molars with probing pocket depths of 4-6 mm. Biochemical indicators were evaluated at baseline and 12 wk using the suspension array system for eight cytokines and chemokines (interleukin [IL]-1β, IL-1ra, IL-4, IL-6, IL-8, IL-10, monocyte chemoattractant protein-1 and tumor necrosis factor [TNF]-α) in gingival crevicular fluid. Results: The levels of IL-1β, IL-6, IL-8 and TNF-α remained significantly lower in the test group compared to the placebo group. In the placebo group, when the probing pocket depth at baseline was 4 mm, IL-1β increased, particularly in the second molar tooth, and the greatest increase was seen when PPD at baseline was 5-6 mm. In the test group, IL-1β decreased markedly in cases with furcation involvement and low bleeding on probing at baseline. In both groups, IL-1β, IL-6 and TNF-α were closely correlated with each other. Conclusion: This OTC medication is biochemically effective for steady chronic periodontitis in the supportive periodontal therapy phase.

KW - Inflammatory cytokine

KW - Over-the-counter medication

KW - Self-medication

KW - Supportive periodontal therapy

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U2 - 10.1111/jre.12311

DO - 10.1111/jre.12311

M3 - Article

JO - Journal of Periodontal Research

JF - Journal of Periodontal Research

SN - 0022-3484

ER -