Effects of metabolic fragments of [Arg8]-vasopressin on nerve growth in cultured hippocampal neurons

Tadatsugu Tarumi, Yukio Sugimoto, Zhong Chen, Qiue Zhao, Chiaki Kamei

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effects of metabolic fragments of [Arg8]-vasopressin (AVP), [pGlu4, Cyt6]AVP (AVP4-9), and desglycinamide-[pGlu4, Cyt6]AVP (AVP4-8) on the growth of hippocampal neurons in culture were investigated in comparison with those of AVP. AVP4-9 caused a significant increase in filopodial length following 96 h of exposure at concentrations higher than 300 nM. AVP4-9 was more potent than AVP. AVP4-8 also induced an increase in filopodial length, but this effect was less than that of AVP. The selective V1 agonist [Phe2, Ile3, Orn8]-vasopressin caused a significant increase in filopodial length, whereas the selective V2 agonist [deamino-Cys1, D-Arg8]-vasopressin showed no such effect. OPC-21268, a vasopressin V1 antagonist, blocked AVP and AVP fragment-induced increases in filopodial length. However, the V2 antagonist OPC-31260 showed no such effect. A23187, a representative Ca ionophore, also increased filopodial length, and the A23187-induced increase in filopodial length was potentiated by AVP and AVP fragments. These results indicated that AVP4-9 and AVP4-8 increased filopodial length in cultured hippocampal neurons by activating V1 receptors. Both phenomena induced by AVP4-9 and AVP4-8 were associated with intracellular calcium mobilization. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)407-411
Number of pages5
JournalBrain Research Bulletin
Volume51
Issue number5
DOIs
Publication statusPublished - Apr 1 2000

Keywords

  • Cultured hippocampal neuron
  • Cyt]AVP
  • Desglycinamide-[pGlu
  • Nerve growth
  • [Arg]-vasopressin
  • [pGlu

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Effects of metabolic fragments of [Arg<sup>8</sup>]-vasopressin on nerve growth in cultured hippocampal neurons'. Together they form a unique fingerprint.

  • Cite this