Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers

Yukitaka Kimura, Yasuo Hosoda, Masayo Yamaguchi, Hiroshi Nagano, Motohiro Shima, Shuji Adachi, Ryuichi Matsuno

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effects of medium-chain fatty acids (MCFA) on intracellular calcium (Ca2+) levels and actin filaments in the Caco-2 monolayer were investigated. A site-dependent increase in intracellular Ca2+ levels caused by decanoic acid (C10) at 13 mM was observed by confocal laser scanning microscopy. The area in which the intracellular Ca2+ levels was increased was measured by image analysis, and increased to 11% of the total area of the monolayer within 1 minute. This was maintained for 5 minutes, and decreased thereafter. The other MC-FAs did not significantly increase the intracellular Ca2+ levels. Obvious morphological changes of actin filaments were induced by only C10 among C8-C14. The area in which actin filaments were depleted was also quantified, and the increase in area became significant after 40 minutes. The area of the actin-depleted spot corresponded to the area occupied by 5 to 10 cells as well as that in which the intracellular Ca2+ level was increased. The effectiveness of only C10 suggested that the mechanism of the absorption enhancement by C10 would be different from that by the other MCFAs, or that C10 has some additional physiological functions although the mechanism of the enhancement is the same as for the other MCFAs.

Original languageEnglish
Pages (from-to)743-751
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number4
DOIs
Publication statusPublished - Apr 2001
Externally publishedYes

Fingerprint

medium chain fatty acids
Caco-2 Cells
cytoskeleton
Cytoskeleton
Actin Cytoskeleton
Fatty acids
Actins
Calcium
Monolayers
Fatty Acids
calcium
microfilaments
Image analysis
Microscopic examination
cells
Scanning
Confocal Microscopy
Acids
Lasers
decanoic acid

Keywords

  • Absorption enhancement
  • Actin filaments
  • Caco-2
  • Intracellular calcium levels
  • Medium chain fatty acid

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers. / Kimura, Yukitaka; Hosoda, Yasuo; Yamaguchi, Masayo; Nagano, Hiroshi; Shima, Motohiro; Adachi, Shuji; Matsuno, Ryuichi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 4, 04.2001, p. 743-751.

Research output: Contribution to journalArticle

Kimura, Yukitaka ; Hosoda, Yasuo ; Yamaguchi, Masayo ; Nagano, Hiroshi ; Shima, Motohiro ; Adachi, Shuji ; Matsuno, Ryuichi. / Effects of medium-chain fatty acids on intracellular calcium levels and the cytoskeleton in human intestinal (Caco-2) cell monolayers. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 4. pp. 743-751.
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