Abstract
Objective: Endothelial cells are exposed to a variety of mechanical stresses, which modulate a number of endothelial functions. One of the important functions of endothelial cells is their migrating ability displayed at healing of vascular injury and angiogenesis. The present study aimed to elucidate the effects of mechanical stresses on the migrating behavior of bovine aorta endothelial cells (BAECs). Methods: BAECs were cultured on a fibronectin-coated elastic silicone membrane. A narrow scar (ca. 200 μ m in width) was made by a scratch in the confluent monolayer of BAECs, and migrating behavior of remaining cells into the vacant area was measured under controlled mechanical stresses onto the cells. Results: When the silicone membrane was continuously stretched by 20% at a stroke perpendicular to the scar, cell migration was strongly accelerated. In contrast, when a 20% prestretched silicone membrane in the same axis as above was relaxed, which effectively generated compressive force onto the cells, cell migration toward the vacant area was significantly inhibited. When mechanical stresses were applied parallel to the scar, cell migration was accelerated moderately by either stretch or compression. Direction of migration and lamellipodia formation was also affected significantly by mechanical stresses. Conclusion: Migrating behavior of BAECs is influenced not only by the modes (stretch or compression) of the applied mechanical stress but also by its orientation (perpendicular or parallel to the scar).
| Original language | English |
|---|---|
| Title of host publication | Biomechanics at Micro- and Nanoscale Levels: Volume I |
| Publisher | World Scientific Publishing Co. |
| Pages | 75-87 |
| Number of pages | 13 |
| ISBN (Electronic) | 9789812569301 |
| ISBN (Print) | 981256098X, 9789812560988 |
| DOIs | |
| Publication status | Published - Jan 1 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Engineering(all)
- Immunology and Microbiology(all)
- Medicine(all)
Cite this
Effects of mechanical stresses on the migrating behavior of endothelial cells. / Tanaka, T.; Naruse, Keiji; Sokabe, Masahir.
Biomechanics at Micro- and Nanoscale Levels: Volume I. World Scientific Publishing Co., 2005. p. 75-87.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Effects of mechanical stresses on the migrating behavior of endothelial cells
AU - Tanaka, T.
AU - Naruse, Keiji
AU - Sokabe, Masahir
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Objective: Endothelial cells are exposed to a variety of mechanical stresses, which modulate a number of endothelial functions. One of the important functions of endothelial cells is their migrating ability displayed at healing of vascular injury and angiogenesis. The present study aimed to elucidate the effects of mechanical stresses on the migrating behavior of bovine aorta endothelial cells (BAECs). Methods: BAECs were cultured on a fibronectin-coated elastic silicone membrane. A narrow scar (ca. 200 μ m in width) was made by a scratch in the confluent monolayer of BAECs, and migrating behavior of remaining cells into the vacant area was measured under controlled mechanical stresses onto the cells. Results: When the silicone membrane was continuously stretched by 20% at a stroke perpendicular to the scar, cell migration was strongly accelerated. In contrast, when a 20% prestretched silicone membrane in the same axis as above was relaxed, which effectively generated compressive force onto the cells, cell migration toward the vacant area was significantly inhibited. When mechanical stresses were applied parallel to the scar, cell migration was accelerated moderately by either stretch or compression. Direction of migration and lamellipodia formation was also affected significantly by mechanical stresses. Conclusion: Migrating behavior of BAECs is influenced not only by the modes (stretch or compression) of the applied mechanical stress but also by its orientation (perpendicular or parallel to the scar).
AB - Objective: Endothelial cells are exposed to a variety of mechanical stresses, which modulate a number of endothelial functions. One of the important functions of endothelial cells is their migrating ability displayed at healing of vascular injury and angiogenesis. The present study aimed to elucidate the effects of mechanical stresses on the migrating behavior of bovine aorta endothelial cells (BAECs). Methods: BAECs were cultured on a fibronectin-coated elastic silicone membrane. A narrow scar (ca. 200 μ m in width) was made by a scratch in the confluent monolayer of BAECs, and migrating behavior of remaining cells into the vacant area was measured under controlled mechanical stresses onto the cells. Results: When the silicone membrane was continuously stretched by 20% at a stroke perpendicular to the scar, cell migration was strongly accelerated. In contrast, when a 20% prestretched silicone membrane in the same axis as above was relaxed, which effectively generated compressive force onto the cells, cell migration toward the vacant area was significantly inhibited. When mechanical stresses were applied parallel to the scar, cell migration was accelerated moderately by either stretch or compression. Direction of migration and lamellipodia formation was also affected significantly by mechanical stresses. Conclusion: Migrating behavior of BAECs is influenced not only by the modes (stretch or compression) of the applied mechanical stress but also by its orientation (perpendicular or parallel to the scar).
UR - http://www.scopus.com/inward/record.url?scp=84967638101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84967638101&partnerID=8YFLogxK
U2 - 10.1142/9789812569301_0007
DO - 10.1142/9789812569301_0007
M3 - Chapter
AN - SCOPUS:84967638101
SN - 981256098X
SN - 9789812560988
SP - 75
EP - 87
BT - Biomechanics at Micro- and Nanoscale Levels: Volume I
PB - World Scientific Publishing Co.
ER -