Effects of mechanical stimulation by a powered toothbrush on the healing of periodontal tissue in a rat model of periodontal disease

Daisuke Ekuni, Reiko Yamanaka, T. Yamamoto, M. Miyauchi, T. Takata, T. Watanabe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and Objective: Elimination of pathogens is the main aim of periodontal treatment; however, modulation of the host immune response should also be considered. This study aimed to evaluate the effects of mechanical stimulation on periodontal healing in rats. Material and Methods: Before starting the experiment, lipopolysaccharide and proteases were applied once a day, for 4 wk, to both maxillary first molars of 30 rats to induce periodontal disease, and the application was stopped at the end of the 4-wk period. The experiment started immediately following this pretreatment. In the experiment, the left palatal gingiva was stimulated once daily using a powered toothbrush and the right gingiva served as a control (no mechanical stimulation). Pathological changes, and proliferation and cell death in periodontal tissues, were evaluated histometrically and immunohistochemically at baseline (0 wk), and at 1 and 3 wk of stimulation. Results: The control showed a reduction of polymorphonuclear leukocyte infiltration in connective tissue and an increase in the numbers of gingival and periodontal ligament fibroblasts. Mechanical stimulation reduced polymorphonuclear leukocyte infiltration and the area of destroyed collagen in connective tissue, and increased the number of gingival fibroblasts; however, it had no effect on alveolar bone and root resorption or on the number of periodontal ligament fibroblasts. Conclusion: Mechanical stimulation accelerated the healing of gingival inflammation by reducing the infiltration of polymorphonuclear leukocytes and enhancing fibroblast proliferation and collagen synthesis.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalJournal of Periodontal Research
Volume45
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Periodontal Diseases
Fibroblasts
Periodontal Ligament
Neutrophils
Gingiva
Connective Tissue
Collagen
Root Resorption
Alveolar Bone Loss
Bone Resorption
Lipopolysaccharides
Peptide Hydrolases
Cell Death
Inflammation

Keywords

  • Collagen
  • Fibroblasts
  • Mechanical stimulation
  • Periodontal disease
  • Proliferating cell nuclear antigen
  • Rat

ASJC Scopus subject areas

  • Periodontics

Cite this

Effects of mechanical stimulation by a powered toothbrush on the healing of periodontal tissue in a rat model of periodontal disease. / Ekuni, Daisuke; Yamanaka, Reiko; Yamamoto, T.; Miyauchi, M.; Takata, T.; Watanabe, T.

In: Journal of Periodontal Research, Vol. 45, No. 1, 02.2010, p. 45-51.

Research output: Contribution to journalArticle

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abstract = "Background and Objective: Elimination of pathogens is the main aim of periodontal treatment; however, modulation of the host immune response should also be considered. This study aimed to evaluate the effects of mechanical stimulation on periodontal healing in rats. Material and Methods: Before starting the experiment, lipopolysaccharide and proteases were applied once a day, for 4 wk, to both maxillary first molars of 30 rats to induce periodontal disease, and the application was stopped at the end of the 4-wk period. The experiment started immediately following this pretreatment. In the experiment, the left palatal gingiva was stimulated once daily using a powered toothbrush and the right gingiva served as a control (no mechanical stimulation). Pathological changes, and proliferation and cell death in periodontal tissues, were evaluated histometrically and immunohistochemically at baseline (0 wk), and at 1 and 3 wk of stimulation. Results: The control showed a reduction of polymorphonuclear leukocyte infiltration in connective tissue and an increase in the numbers of gingival and periodontal ligament fibroblasts. Mechanical stimulation reduced polymorphonuclear leukocyte infiltration and the area of destroyed collagen in connective tissue, and increased the number of gingival fibroblasts; however, it had no effect on alveolar bone and root resorption or on the number of periodontal ligament fibroblasts. Conclusion: Mechanical stimulation accelerated the healing of gingival inflammation by reducing the infiltration of polymorphonuclear leukocytes and enhancing fibroblast proliferation and collagen synthesis.",
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