Effects of low-intensity pulsed ultrasound on osteoclasts

Analysis with goldfish scales as a model of bone

Taizo Hanmoto, Yoshiaki Tabuchi, Mika Ikegame, Takashi Kondo, Kei Ichiro Kitamura, Masato Endo, Isao Kobayashi, Hiroyuki Mishima, Toshio Sekiguchi, Makoto Urata, Azusa Seki, Sachiko Yano, Atsuhiko Hattori, Nobuo Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes’ mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalBiomedical Research (Japan)
Volume38
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Goldfish
Osteoclasts
Bone
Ultrasonics
Bone and Bones
Messenger RNA
Osteogenesis
Osteoprotegerin
Osteoblasts
Genes
Gene Expression
Enzymes
Transcription
Fish
Regeneration
Fishes
Transcription Factors
Ultrasonic Waves
Ligands
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of low-intensity pulsed ultrasound on osteoclasts : Analysis with goldfish scales as a model of bone. / Hanmoto, Taizo; Tabuchi, Yoshiaki; Ikegame, Mika; Kondo, Takashi; Kitamura, Kei Ichiro; Endo, Masato; Kobayashi, Isao; Mishima, Hiroyuki; Sekiguchi, Toshio; Urata, Makoto; Seki, Azusa; Yano, Sachiko; Hattori, Atsuhiko; Suzuki, Nobuo.

In: Biomedical Research (Japan), Vol. 38, No. 1, 2017, p. 71-77.

Research output: Contribution to journalArticle

Hanmoto, T, Tabuchi, Y, Ikegame, M, Kondo, T, Kitamura, KI, Endo, M, Kobayashi, I, Mishima, H, Sekiguchi, T, Urata, M, Seki, A, Yano, S, Hattori, A & Suzuki, N 2017, 'Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone', Biomedical Research (Japan), vol. 38, no. 1, pp. 71-77. https://doi.org/10.2220/biomedres.38.71
Hanmoto, Taizo ; Tabuchi, Yoshiaki ; Ikegame, Mika ; Kondo, Takashi ; Kitamura, Kei Ichiro ; Endo, Masato ; Kobayashi, Isao ; Mishima, Hiroyuki ; Sekiguchi, Toshio ; Urata, Makoto ; Seki, Azusa ; Yano, Sachiko ; Hattori, Atsuhiko ; Suzuki, Nobuo. / Effects of low-intensity pulsed ultrasound on osteoclasts : Analysis with goldfish scales as a model of bone. In: Biomedical Research (Japan). 2017 ; Vol. 38, No. 1. pp. 71-77.
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