Effects of lincomycin on synthesis of TEM β-lactamase by Escherichia coli

A. Okabe, O. Matsushita, Osamu Matsushita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sub-inhibitory concentrations of lincomycin slightly inhibit growth of Escherichia coli carrying plasmid RP4 and cause a 2-fold increase in TEM-2 β-lactamase. To analyze this effect, cultures were pulse-labeled with [3H]leucine, chased with non-radioactive leucine and immunoprecipitated with anti-β-lactamase antiserum. The synthesis rate of β-lactamase was two times higher in inhibited cultures than in control cultures. No significant decrease of labeled enzyme occurred during the 30 minutes chase, indicating no degradation of β-lactamase. The rate of maturation of pre-β-lactamase was determined by measuring the decrease in the amount of pre-β-lactamase after a 1-minute labeling interval. There was no significant difference between the control and lincomycin-treated cultures, indicating that posttranslational translocation is not involved in the stimulation. Both plasmid encoded and chromosomally encoded TEM-1 β-lactamase increased in the presence of lincomycin. The effects of other protein synthesis inhibitors on the synthesis of TEM-1 β-lactamase were examined. The stimulation of β-lactamase synthesis by linocmycin appears to be specific for macrolide and related antibiotics and is not a general phenomenon resulting from partial inhibition of protein synthesis.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalJournal of Antibiotics
Volume41
Issue number5
Publication statusPublished - 1988

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Lincomycin
Escherichia coli
Leucine
Plasmids
Protein Synthesis Inhibitors
Macrolides
Immune Sera
Anti-Bacterial Agents
Enzymes
Growth
Proteins

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Effects of lincomycin on synthesis of TEM β-lactamase by Escherichia coli. / Okabe, A.; Matsushita, O.; Matsushita, Osamu.

In: Journal of Antibiotics, Vol. 41, No. 5, 1988, p. 667-674.

Research output: Contribution to journalArticle

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