Effects of Lectins on initial attachment of cariogenic Streptococcus mutans

Takashi Ito, Yasuhiro Yoshida, Yasuyoshi Shiota, Yuki Ito, Tadashi Yamamoto, Shogo Takashiba

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Oral bacteria initiate biofilm formation by attaching to tooth surfaces via an interaction of a lectin-like bacterial protein with carbohydrate chains on the pellicle. This study aimed to find naturally derived lectins that inhibit the initial attachment of a cariogenic bacterial species, Streptococcus mutans (S. mutans), to carbohydrate chains in saliva in vitro. Seventy kinds of lectins were screened for candidate motifs that inhibit the attachment of S. mutans ATCC 25175 to a saliva-coated culture plate. The inhibitory effect of the lectins on attachment of the S. mutans to the plates was quantified by crystal violet staining, and the biofilm was observed under a scanning electron microscope (SEM). Surface plasmon resonance (SPR) analysis was performed to examine the binding of S. mutans to carbohydrate chains and the binding of candidate lectins to carbohydrate chains, respectively. Moreover, binding assay between the biotinylated-lectins and the saliva components was conducted to measure the lectin binding. Lectins recognizing a salivary carbohydrate chain, Galβ1-3GalNAc, inhibited the binding of S. mutans to the plate. In particular, Agaricus bisporus agglutinin (ABA) markedly inhibited the binding. This inhibition was confirmed by SEM observation. SPR analysis indicated that S. mutans strongly binds to Galβ1-3GalNAc, and ABA binds to Galβ1-3GalNAc. Finally, the biotinylated Galβ1-3GalNAc-binding lectins including ABA demonstrated marked binding to the saliva components. These results suggest that ABA lectin inhibited the attachment of S. mutans to Galβ1-3GalNAc in saliva and ABA can be useful as a potent inhibitor for initial attachment of oral bacteria and biofilm formation.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalGlycoconjugate Journal
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Streptococcus mutans
Lectins
Agglutinins
Saliva
Agaricus
Carbohydrates
Biofilms
Surface Plasmon Resonance
Surface plasmon resonance
Electrons
Bacteria
Gentian Violet
Electron microscopes
Bacterial Proteins
Scanning
Tooth
Observation
Staining and Labeling
Assays

Keywords

  • Biofilm
  • Carbohydrate chains
  • Inhibitor
  • Initial attachment
  • Lectin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Effects of Lectins on initial attachment of cariogenic Streptococcus mutans. / Ito, Takashi; Yoshida, Yasuhiro; Shiota, Yasuyoshi; Ito, Yuki; Yamamoto, Tadashi; Takashiba, Shogo.

In: Glycoconjugate Journal, 01.02.2018, p. 1-11.

Research output: Contribution to journalArticle

Ito, Takashi ; Yoshida, Yasuhiro ; Shiota, Yasuyoshi ; Ito, Yuki ; Yamamoto, Tadashi ; Takashiba, Shogo. / Effects of Lectins on initial attachment of cariogenic Streptococcus mutans. In: Glycoconjugate Journal. 2018 ; pp. 1-11.
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