Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells

Keiji Matsuda; Shin Ya Nakagawa; Tomoya Nakano; Jun Ichi Asaumi; Ganesh Chandra Jagetia; Shoji Kawasaki

doi:10.1080/02656730601074375

Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells

Keiji Matsuda, Shin Ya Nakagawa, Tomoya Nakano, Jun Ichi Asaumi, Ganesh Chandra Jagetia, Shoji Kawasaki

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

Original language	English
Pages (from-to)	729-735
Number of pages	7
Journal	International Journal of Hyperthermia
Volume	22
Issue number	8
DOIs	https://doi.org/10.1080/02656730601074375
Publication status	Published - Dec 2006

Keywords

H3-Lys4 methylation
H3-Lys9 methylation
HSPs
Hyperthermia
KNK437

ASJC Scopus subject areas

Physiology
Physiology (medical)
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/02656730601074375

Cite this

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title = "Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells",

abstract = "Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.",

keywords = "H3-Lys4 methylation, H3-Lys9 methylation, HSPs, Hyperthermia, KNK437",

author = "Keiji Matsuda and Nakagawa, {Shin Ya} and Tomoya Nakano and Asaumi, {Jun Ichi} and Jagetia, {Ganesh Chandra} and Shoji Kawasaki",

note = "Funding Information: KNK437 was provided by Kaneka Corporation, Osaka, Japan. This study was supported by a Grant-in-Aid (15591277) for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. Dr G. C. Jagetia was supported by JSPS fellowship, Japan.",

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doi = "10.1080/02656730601074375",

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T1 - Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells

AU - Matsuda, Keiji

AU - Nakagawa, Shin Ya

AU - Nakano, Tomoya

AU - Asaumi, Jun Ichi

AU - Jagetia, Ganesh Chandra

AU - Kawasaki, Shoji

N1 - Funding Information: KNK437 was provided by Kaneka Corporation, Osaka, Japan. This study was supported by a Grant-in-Aid (15591277) for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. Dr G. C. Jagetia was supported by JSPS fellowship, Japan.

PY - 2006/12

Y1 - 2006/12

N2 - Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

AB - Purpose: Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe. Methods: Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437. Results: Heating of HSC4 cells at 45°C for 20 min and KB cells for 3min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 μM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70. Conclusions: The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

KW - H3-Lys4 methylation

KW - H3-Lys9 methylation

KW - HSPs

KW - Hyperthermia

KW - KNK437

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Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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