Effects of intraperitoneally administered l-histidine on food intake, taste, and visceral sensation in rats

Yuka Okusya, Yoshiyuki Hirai, Hitoshi Maezawa, Kazunari Hisadome, Nobuo Inoue, Yutaka Yamazaki, Makoto Funahashi

Research output: Contribution to journalArticle

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Abstract

To evaluate relative factors for anorectic effects of l-histidine, we performed behavioral experiments for measuring food and fluid intake, conditioned taste aversion (CTA), taste disturbance, and c-Fos immunoreactive (Fos-ir) cells before and after i.p. injection with l-histidine in rats. Animals were injected with saline (9 ml/kg, i.p.) for a control group, and saline (9 ml/kg, i.p.) containing l-histidine (0.75, 1.5, 2.0 g/kg) for a l-histidine group. Injection of l-histidine decreased the average value of food intake, and statistically significant anorectic effects were found in animals injected with 1.5 or 2.0 g/kg l-histidine but not with 0.75 g/kg l-histidine. Taste abnormalities were not detected in any of the groups. Animals injected with 2.0 g/kg l-histidine were revealed to present with nausea by the measurement of CTA. In this group, a significant increase in the number of Fos-ir cells was detected both in the area postrema and the nucleus tractus solitarius (NTS). In the 0.75 g/kg l-histidine group, a significant increase in the number of Fos-ir cells was detected only in the NTS. When the ventral gastric branch vagotomy was performed, recovery from anorexia became faster than the sham-operated group, however, vagotomized rats injected with 2.0 g/kg l-histidine still acquired CTA. These data indicate that acute anorectic effects induced by highly concentrated l-histidine are partly caused by induction of nausea and/or visceral discomfort accompanied by neuronal activities in the NTS and the area postrema. We suggest that acute and potent effects of l-histidine on food intake require substantial amount of l-histidine in the diet.

Original languageEnglish
Pages (from-to)467-474
Number of pages8
JournalJournal of Physiological Sciences
Volume67
Issue number4
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Histidine
Eating
Appetite Depressants
Solitary Nucleus
Area Postrema
Nausea
Injections
Vagotomy
Anorexia
Stomach
Diet

Keywords

  • Anorexia
  • Area postrema
  • CTA
  • l-histidine
  • NTS
  • Rats
  • Visceral sensation

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of intraperitoneally administered l-histidine on food intake, taste, and visceral sensation in rats. / Okusya, Yuka; Hirai, Yoshiyuki; Maezawa, Hitoshi; Hisadome, Kazunari; Inoue, Nobuo; Yamazaki, Yutaka; Funahashi, Makoto.

In: Journal of Physiological Sciences, Vol. 67, No. 4, 01.07.2017, p. 467-474.

Research output: Contribution to journalArticle

Okusya, Yuka ; Hirai, Yoshiyuki ; Maezawa, Hitoshi ; Hisadome, Kazunari ; Inoue, Nobuo ; Yamazaki, Yutaka ; Funahashi, Makoto. / Effects of intraperitoneally administered l-histidine on food intake, taste, and visceral sensation in rats. In: Journal of Physiological Sciences. 2017 ; Vol. 67, No. 4. pp. 467-474.
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