Effects of intermittent high glucose on BeWo choriocarcinoma cells in culture.

Akio Masumoto, Norio Takamoto, Hisashi Masuyama, Yoichiro Akahori, Seiji Inoue, Yuji Hiramatsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of this study was to investigate the cellular effects of intermittent high glucose on the human BeWo placental choriocarcinoma cell line, used as a model of the effects of glucose fluctuation in diabetic pregnancies. BeWo cells were subjected to three different glucose conditions for 48 h: 7 mmol/L (control), 42 mmol/L (high glucose), or 7 and 42 mmol/L glucose (intermittent, alternated every 6 h). Cell viability was assessed using cell counts, a cell proliferation assay, and a cell viability assay. Apoptosis was also studied using a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and by immunocytochemistry of fractin, the N-terminal fragment of actin, which can distinguish apoptotic from necrotic cells. Furthermore, the expression of the well-known survival factors of trophoblast cells, heparin-binding epidermal growth factor-like growth factor and leptin, was evaluated by real-time polymerase chain reaction and Western blot analyses. Intermittent high-glucose conditions significantly decreased cell viability and enhanced apoptosis compared with control or continuous high-glucose conditions. Furthermore, the expression of heparin-binding epidermal growth factor-like growth factor, but not that of leptin, was significantly increased under intermittent high-glucose conditions compared to its expression under either control or continuous high-glucose conditions. These data indicate that intermittent high glucose is more deleterious to BeWo cells than continuous high-glucose conditions. Although further in vitro and in vivo study is necessary, excess fluctuation of glucose levels in the placental circulation might be involved in the impairment of placental development leading to the placental dysfunction.

Original languageEnglish
Pages (from-to)1365-1375
Number of pages11
JournalJournal of Obstetrics and Gynaecology Research
Volume37
Issue number10
Publication statusPublished - Oct 2011
Externally publishedYes

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Choriocarcinoma
Cell Culture Techniques
Glucose
Cell Survival
Leptin
Epidermal Growth Factor
Heparin
Intercellular Signaling Peptides and Proteins
Placental Circulation
Apoptosis
Pregnancy in Diabetics
Placentation
DNA Nucleotidylexotransferase
Trophoblasts
Actins
Real-Time Polymerase Chain Reaction
Cell Count
Western Blotting
Immunohistochemistry

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Masumoto, A., Takamoto, N., Masuyama, H., Akahori, Y., Inoue, S., & Hiramatsu, Y. (2011). Effects of intermittent high glucose on BeWo choriocarcinoma cells in culture. Journal of Obstetrics and Gynaecology Research, 37(10), 1365-1375.

Effects of intermittent high glucose on BeWo choriocarcinoma cells in culture. / Masumoto, Akio; Takamoto, Norio; Masuyama, Hisashi; Akahori, Yoichiro; Inoue, Seiji; Hiramatsu, Yuji.

In: Journal of Obstetrics and Gynaecology Research, Vol. 37, No. 10, 10.2011, p. 1365-1375.

Research output: Contribution to journalArticle

Masumoto, A, Takamoto, N, Masuyama, H, Akahori, Y, Inoue, S & Hiramatsu, Y 2011, 'Effects of intermittent high glucose on BeWo choriocarcinoma cells in culture.', Journal of Obstetrics and Gynaecology Research, vol. 37, no. 10, pp. 1365-1375.
Masumoto, Akio ; Takamoto, Norio ; Masuyama, Hisashi ; Akahori, Yoichiro ; Inoue, Seiji ; Hiramatsu, Yuji. / Effects of intermittent high glucose on BeWo choriocarcinoma cells in culture. In: Journal of Obstetrics and Gynaecology Research. 2011 ; Vol. 37, No. 10. pp. 1365-1375.
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