Effects of interleukin-8 on estradiol and progesterone production by bovine granulosa cells from large follicles and progesterone production by luteinizing granulosa cells in culture

Takashi Shimizu, Ayami Kaji, Chiaki Murayama, Fumie Magata, Koumei Shirasuna, Kaori Wakamiya, Kiyoshi Okuda, Akio Miyamoto

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Interleukin 8 (IL-8) is a chemoattractant involved in the recruitment and activation of neutrophils and is associated with the ovulate process. We examined the possible role of IL-8 in steroid production by bovine granulosa cells before and after ovulation. The concentration of IL-8 in the follicular fluid of estrogen-active dominant (EAD) and pre-ovulatory follicles (POF) was higher than that of small follicles (SF). CXCR1 mRNA expression was higher in the granulosa cells of EAD and POF than that of SF. In contrast, CXCR2 mRNA expression was lower in granulosa cells of EAD and POF than in SF. IL-8 inhibited estradiol (E2) production in follicle-stimulating hormone (FSH)-treated granulosa cells at 48. h of culture. IL-8 also suppressed CYP19A1 mRNA expression in FSH-treated granulosa cells. IL-8 stimulated progesterone (P4) production in luteinizing hormone (LH)-treated granulosa cells at 48. h of culture. Although IL-8 did not alter the expression of genes associated with P4 production, it induced StAR protein expression in LH-treated granulosa cells. The expression of CXCR1 mRNA in corpus luteum (CL) did not change during the luteal phase. In contrast, the expression of CXCR2 mRNA in middle CL was significantly higher than in early and regression CL during the luteal phase. In luteinizing granulosa cells, an in vitro model of granulosa cell luteinization, CXCR2 mRNA expression was downregulated, whereas CXCR1 mRNA expression was unchanged. IL-8 also stimulated P4 production in luteinizing granulosa cells. These data provide evidence that IL-8 functions not only as a chemokine, but also act as a regulator of steroid synthesis in granulosa cells to promote luteinization after ovulation.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalCytokine
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Granulosa Cells
Interleukin-8
Progesterone
Estradiol
Cell Culture Techniques
Messenger RNA
Estrogens
Luteinization
Follicle Stimulating Hormone
Luteinizing Hormone
Luteal Phase
Corpus Luteum
Ovulation
Steroids
Chemotactic Factors
Luteolysis
Follicular Fluid
Neutrophil Activation
Chemokines
Neutrophil Infiltration

Keywords

  • Granulosa cells
  • IL-8
  • Steroid hormone

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Effects of interleukin-8 on estradiol and progesterone production by bovine granulosa cells from large follicles and progesterone production by luteinizing granulosa cells in culture. / Shimizu, Takashi; Kaji, Ayami; Murayama, Chiaki; Magata, Fumie; Shirasuna, Koumei; Wakamiya, Kaori; Okuda, Kiyoshi; Miyamoto, Akio.

In: Cytokine, Vol. 57, No. 1, 01.2012, p. 175-181.

Research output: Contribution to journalArticle

Shimizu, Takashi ; Kaji, Ayami ; Murayama, Chiaki ; Magata, Fumie ; Shirasuna, Koumei ; Wakamiya, Kaori ; Okuda, Kiyoshi ; Miyamoto, Akio. / Effects of interleukin-8 on estradiol and progesterone production by bovine granulosa cells from large follicles and progesterone production by luteinizing granulosa cells in culture. In: Cytokine. 2012 ; Vol. 57, No. 1. pp. 175-181.
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