Effects of interleukin-31 on MUC5AC gene expression in nasal allergic inflammation

Said Ahmad Shah, Hajime Ishinaga, Bo Hou, Mitsuhiro Okano, Kazuhiko Takeuchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Interleukin-31 (IL-31) is a newly discovered T helper lymphocyte-derived cytokine that plays an important role in allergic inflammation. However, the effects of IL-31 on mucus production in nasal allergic inflammation are completely unknown. Objective: To investigate the effects of IL-31 on mucin gene expression (MUC5AC) in patients with allergic rhinitis and in human airway epithelial cells. Methods: Expression levels of IL-31 and IL-31 receptor A (IL-31RA) were evaluated in the inferior turbinate of patients with allergic rhinitis and non-allergic rhinitis with immunohistochemistry. IL-31-induced MUC5AC gene expression was measured with a MUC5AC luciferase reporter assay in human epithelial HM3-MUC5AC cells and quantified by quantitative real-time polymerase chain reaction in human airway epithelial A549 cells. Results: IL-31RA was primarily localized in submucosal glands and upregulated in allergic rhinitis. IL-31 was detected in submucosal tissue and increased in allergic inflammation. MUC5AC gene expression was induced by IL-31 stimulation both in HM3-MUC5AC and A549 cells. Additionally, IL-31 cooperated with Th2 cytokines on MUC5AC gene expression in HM3-MUC5AC cells. Conclusion: IL-31 and IL-31RA are upregulated in patients with allergic rhinitis, and induce MUC5AC gene expression in human airway epithelial cells. These findings suggest that IL-31 plays an important role in mucus overproduction in nasal allergic inflammation.

Original languageEnglish
Pages (from-to)158-164
Number of pages7
JournalPharmacology
Volume91
Issue number3-4
DOIs
Publication statusPublished - May 2013

Fingerprint

Interleukins
Nose
Inflammation
Gene Expression
Epithelial Cells
Mucus
Interleukin Receptors
Cytokines
Turbinates
Mucins
Rhinitis
Helper-Inducer T-Lymphocytes
Luciferases
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Allergic Rhinitis

Keywords

  • Allergic rhinitis
  • Interleukin-31
  • MUC5AC
  • Th2 cytokines

ASJC Scopus subject areas

  • Pharmacology

Cite this

Shah, S. A., Ishinaga, H., Hou, B., Okano, M., & Takeuchi, K. (2013). Effects of interleukin-31 on MUC5AC gene expression in nasal allergic inflammation. Pharmacology, 91(3-4), 158-164. https://doi.org/10.1159/000346609

Effects of interleukin-31 on MUC5AC gene expression in nasal allergic inflammation. / Shah, Said Ahmad; Ishinaga, Hajime; Hou, Bo; Okano, Mitsuhiro; Takeuchi, Kazuhiko.

In: Pharmacology, Vol. 91, No. 3-4, 05.2013, p. 158-164.

Research output: Contribution to journalArticle

Shah, SA, Ishinaga, H, Hou, B, Okano, M & Takeuchi, K 2013, 'Effects of interleukin-31 on MUC5AC gene expression in nasal allergic inflammation', Pharmacology, vol. 91, no. 3-4, pp. 158-164. https://doi.org/10.1159/000346609
Shah, Said Ahmad ; Ishinaga, Hajime ; Hou, Bo ; Okano, Mitsuhiro ; Takeuchi, Kazuhiko. / Effects of interleukin-31 on MUC5AC gene expression in nasal allergic inflammation. In: Pharmacology. 2013 ; Vol. 91, No. 3-4. pp. 158-164.
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