Effects of insulin signaling on mouse taste cell proliferation

Shingo Takai, Yu Watanabe, Keisuke Sanematsu, Ryusuke Yoshida, Robert F. Margolskee, Peihua Jiang, Ikiru Atsuta, Kiyoshi Koyano, Yuzo Ninomiya, Noriatsu Shigemura

Research output: Contribution to journalArticle

Abstract

Expression of insulin and its receptor (IR) in rodent taste cells has been proposed, but exactly which types of taste cells express IR and the function of insulin signaling in taste organ have yet to be determined. In this study, we analyzed expression of IR mRNA and protein in mouse taste bud cells in vivo and explored its function ex vivo in organoids, using RT-PCR, immunohistochemistry, and quantitative PCR. In mouse taste tissue, IR was expressed broadly in taste buds, including in type II and III taste cells. With using 3-D taste bud organoids, we found insulin in the culture medium significantly decreased the number of taste cell and mRNA expression levels of many taste cell genes, including nucleoside triphosphate diphosphohydrolase-2 (NTPDase2), Tas1R3 (T1R3), gustducin, carbonic anhydrase 4 (CA4), glucose transporter-8 (GLUT8), and sodium-glucose cotransporter-1 (SGLT1) in a concentration-dependent manner. Rapamycin, an inhibitor of mechanistic target of rapamycin (mTOR) signaling, diminished insulin’s effects and increase taste cell generation. Altogether, circulating insulin might be an important regulator of taste cell growth and/or proliferation via activation of the mTOR pathway.

Original languageEnglish
Article numbere0225190
JournalPloS one
Volume14
Issue number11
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Cell proliferation
Sirolimus
cell proliferation
insulin
Cell Proliferation
Insulin
Carbonic Anhydrase IV
Sodium-Glucose Transporter 1
mice
Taste Buds
Insulins
Messenger RNA
Insulin Receptor
palps
Cell growth
Nucleosides
Organoids
Glucose
Culture Media
cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Takai, S., Watanabe, Y., Sanematsu, K., Yoshida, R., Margolskee, R. F., Jiang, P., ... Shigemura, N. (2019). Effects of insulin signaling on mouse taste cell proliferation. PloS one, 14(11), [e0225190]. https://doi.org/10.1371/journal.pone.0225190

Effects of insulin signaling on mouse taste cell proliferation. / Takai, Shingo; Watanabe, Yu; Sanematsu, Keisuke; Yoshida, Ryusuke; Margolskee, Robert F.; Jiang, Peihua; Atsuta, Ikiru; Koyano, Kiyoshi; Ninomiya, Yuzo; Shigemura, Noriatsu.

In: PloS one, Vol. 14, No. 11, e0225190, 01.01.2019.

Research output: Contribution to journalArticle

Takai, S, Watanabe, Y, Sanematsu, K, Yoshida, R, Margolskee, RF, Jiang, P, Atsuta, I, Koyano, K, Ninomiya, Y & Shigemura, N 2019, 'Effects of insulin signaling on mouse taste cell proliferation', PloS one, vol. 14, no. 11, e0225190. https://doi.org/10.1371/journal.pone.0225190
Takai S, Watanabe Y, Sanematsu K, Yoshida R, Margolskee RF, Jiang P et al. Effects of insulin signaling on mouse taste cell proliferation. PloS one. 2019 Jan 1;14(11). e0225190. https://doi.org/10.1371/journal.pone.0225190
Takai, Shingo ; Watanabe, Yu ; Sanematsu, Keisuke ; Yoshida, Ryusuke ; Margolskee, Robert F. ; Jiang, Peihua ; Atsuta, Ikiru ; Koyano, Kiyoshi ; Ninomiya, Yuzo ; Shigemura, Noriatsu. / Effects of insulin signaling on mouse taste cell proliferation. In: PloS one. 2019 ; Vol. 14, No. 11.
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