Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone

Koji Yachiguchi, Toshio Sekiguchi, Masaki Nakano, Atsuhiko Hattori, Megumi Yamamoto, Kei Ichiro Kitamura, Masahiro Maeda, Yoshiaki Tabuchi, Takashi Kondo, Hiromitsu Kamauchi, Hajime Nakabayashi, Ajai K. Srivastav, Kazuichi Hayakawa, Tatsuya Sakamoto, Nobuo Suzuki

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Abstract

To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10-5 and 10-4 M) and MeHg (10-6 to 10-4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10-5 and 10-4 M) and MeHg (10-6 to 10-4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

Original languageEnglish
Pages (from-to)330-337
Number of pages8
JournalZoological Science
Volume31
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

methylmercury compounds
osteoclasts
osteoblasts
acid phosphatase
mercury
bones
alkaline phosphatase
Girella
bone metabolism
metallothionein
collagen
binding proteins
metals
enzyme activity
fish

Keywords

  • inorganic mercury
  • methylmercury
  • nibbler fish
  • osteoblasts
  • osteoclasts
  • scales

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Yachiguchi, K., Sekiguchi, T., Nakano, M., Hattori, A., Yamamoto, M., Kitamura, K. I., ... Suzuki, N. (2014). Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone. Zoological Science, 31(5), 330-337. https://doi.org/10.2108/zs130265

Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone. / Yachiguchi, Koji; Sekiguchi, Toshio; Nakano, Masaki; Hattori, Atsuhiko; Yamamoto, Megumi; Kitamura, Kei Ichiro; Maeda, Masahiro; Tabuchi, Yoshiaki; Kondo, Takashi; Kamauchi, Hiromitsu; Nakabayashi, Hajime; Srivastav, Ajai K.; Hayakawa, Kazuichi; Sakamoto, Tatsuya; Suzuki, Nobuo.

In: Zoological Science, Vol. 31, No. 5, 2014, p. 330-337.

Research output: Contribution to journalArticle

Yachiguchi, K, Sekiguchi, T, Nakano, M, Hattori, A, Yamamoto, M, Kitamura, KI, Maeda, M, Tabuchi, Y, Kondo, T, Kamauchi, H, Nakabayashi, H, Srivastav, AK, Hayakawa, K, Sakamoto, T & Suzuki, N 2014, 'Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone', Zoological Science, vol. 31, no. 5, pp. 330-337. https://doi.org/10.2108/zs130265
Yachiguchi, Koji ; Sekiguchi, Toshio ; Nakano, Masaki ; Hattori, Atsuhiko ; Yamamoto, Megumi ; Kitamura, Kei Ichiro ; Maeda, Masahiro ; Tabuchi, Yoshiaki ; Kondo, Takashi ; Kamauchi, Hiromitsu ; Nakabayashi, Hajime ; Srivastav, Ajai K. ; Hayakawa, Kazuichi ; Sakamoto, Tatsuya ; Suzuki, Nobuo. / Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone. In: Zoological Science. 2014 ; Vol. 31, No. 5. pp. 330-337.
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AU - Suzuki, Nobuo

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