Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass, whole crop maize, guinea grass and rhodes grass silages

S. Parvin, C. Wang, Y. Li, Naoki Nishino

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Italian ryegrass (IR), whole crop maize (WM), guinea grass (GG) and rhodes grass (RG) were stored in laboratory silos for 30. d with and without Lactobacillus plantarum or Lactobacillus brevis inoculation. The grasses were wilted to low moisture levels (about 500. g/kg) prior to ensiling, while WM was ensiled directly after harvest. Microbial counts and bacterial community analyses were undertaken by plate-culture and denaturing gradient gel electrophoresis (DGGE), respectively, on pre- and post-ensiled crops. A total of 31 DGGE bands were retrieved, cloned, and sequenced to identify bacterial species according to their 16S rRNA gene sequences. Inoculation with L. plantarum and L. brevis increased (P

Original languageEnglish
Pages (from-to)160-166
Number of pages7
JournalAnimal Feed Science and Technology
Volume160
Issue number3-4
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Chloris gayana
Megathyrsus maximus
Lolium multiflorum
grass silage
bacterial communities
lactic acid bacteria
Lactobacillus brevis
denaturing gradient gel electrophoresis
Lactobacillus plantarum
corn
crops
silage making
ribosomal RNA
grasses
nucleotide sequences

Keywords

  • Denaturing gradient gel electrophoresis
  • Fermentation
  • Lactic acid bacteria

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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abstract = "Italian ryegrass (IR), whole crop maize (WM), guinea grass (GG) and rhodes grass (RG) were stored in laboratory silos for 30. d with and without Lactobacillus plantarum or Lactobacillus brevis inoculation. The grasses were wilted to low moisture levels (about 500. g/kg) prior to ensiling, while WM was ensiled directly after harvest. Microbial counts and bacterial community analyses were undertaken by plate-culture and denaturing gradient gel electrophoresis (DGGE), respectively, on pre- and post-ensiled crops. A total of 31 DGGE bands were retrieved, cloned, and sequenced to identify bacterial species according to their 16S rRNA gene sequences. Inoculation with L. plantarum and L. brevis increased (P",
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AU - Wang, C.

AU - Li, Y.

AU - Nishino, Naoki

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