Effects of inhibitors and NaCl on the oxidation of reduced inorganic sulfur compounds by a marine acidophilic, sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH

Kazuo Kamimura, Emi Higashino, Tadayoshi Kanao, Tsuyoshi Sugio

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effect of NaCl and the pathways of the oxidation of reduced inorganic sulfur compounds were studied using resting cells and cell-free extracts of Acidithiobacillus thiooxidans strain SH. This isolate specifically requires NaCl for growth. The oxidation of sulfur and sulfite by resting cells was strongly inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. Carbonylcyanide m-chlorophenyl-hydrazone and monensin were also relatively strong inhibitors. Thiosulfate-oxidizing activity was not inhibited by these uncouplers. Valinomycin did not inhibit the oxidation of sulfur compounds. NaCl stimulated the sulfur- and sulfite-oxidizing activities in resting cells but not in cell-free extracts. The tetrathionate-oxidizing activity in resting cells was slightly stimulated by NaCl, whereas it did not influence the thiosulfate-oxidizing activity. Sulfide oxidation was biphasic, suggesting the formation of intermediate sulfur. The initial phase of sulfide oxidation was not affected by NaCl, whereas the subsequent oxidation of sulfur in the second phase was Na+-dependent. A model is proposed for the role of NaCl in the metabolism of reduced sulfur compounds in A. thiooxidans strain SH.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalExtremophiles
Volume9
Issue number1
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Acidithiobacillus thiooxidans
Sulfur Compounds
Sulfur
Bacteria
Thiosulfates
Sulfites
Sulfides
Cell Extracts
Valinomycin
Hydrazones
Monensin
Growth

Keywords

  • Acidithiobacillus thiooxidans
  • Acidophile
  • Chemolithotroph
  • Halophile
  • Sulfur-oxidizing bacterium

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

@article{c1705c5abcbf46dfb783724d524375d9,
title = "Effects of inhibitors and NaCl on the oxidation of reduced inorganic sulfur compounds by a marine acidophilic, sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH",
abstract = "The effect of NaCl and the pathways of the oxidation of reduced inorganic sulfur compounds were studied using resting cells and cell-free extracts of Acidithiobacillus thiooxidans strain SH. This isolate specifically requires NaCl for growth. The oxidation of sulfur and sulfite by resting cells was strongly inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. Carbonylcyanide m-chlorophenyl-hydrazone and monensin were also relatively strong inhibitors. Thiosulfate-oxidizing activity was not inhibited by these uncouplers. Valinomycin did not inhibit the oxidation of sulfur compounds. NaCl stimulated the sulfur- and sulfite-oxidizing activities in resting cells but not in cell-free extracts. The tetrathionate-oxidizing activity in resting cells was slightly stimulated by NaCl, whereas it did not influence the thiosulfate-oxidizing activity. Sulfide oxidation was biphasic, suggesting the formation of intermediate sulfur. The initial phase of sulfide oxidation was not affected by NaCl, whereas the subsequent oxidation of sulfur in the second phase was Na+-dependent. A model is proposed for the role of NaCl in the metabolism of reduced sulfur compounds in A. thiooxidans strain SH.",
keywords = "Acidithiobacillus thiooxidans, Acidophile, Chemolithotroph, Halophile, Sulfur-oxidizing bacterium",
author = "Kazuo Kamimura and Emi Higashino and Tadayoshi Kanao and Tsuyoshi Sugio",
year = "2005",
month = "2",
doi = "10.1007/s00792-004-0420-5",
language = "English",
volume = "9",
pages = "45--51",
journal = "Extremophiles : life under extreme conditions",
issn = "1431-0651",
publisher = "Springer Japan",
number = "1",

}

TY - JOUR

T1 - Effects of inhibitors and NaCl on the oxidation of reduced inorganic sulfur compounds by a marine acidophilic, sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH

AU - Kamimura, Kazuo

AU - Higashino, Emi

AU - Kanao, Tadayoshi

AU - Sugio, Tsuyoshi

PY - 2005/2

Y1 - 2005/2

N2 - The effect of NaCl and the pathways of the oxidation of reduced inorganic sulfur compounds were studied using resting cells and cell-free extracts of Acidithiobacillus thiooxidans strain SH. This isolate specifically requires NaCl for growth. The oxidation of sulfur and sulfite by resting cells was strongly inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. Carbonylcyanide m-chlorophenyl-hydrazone and monensin were also relatively strong inhibitors. Thiosulfate-oxidizing activity was not inhibited by these uncouplers. Valinomycin did not inhibit the oxidation of sulfur compounds. NaCl stimulated the sulfur- and sulfite-oxidizing activities in resting cells but not in cell-free extracts. The tetrathionate-oxidizing activity in resting cells was slightly stimulated by NaCl, whereas it did not influence the thiosulfate-oxidizing activity. Sulfide oxidation was biphasic, suggesting the formation of intermediate sulfur. The initial phase of sulfide oxidation was not affected by NaCl, whereas the subsequent oxidation of sulfur in the second phase was Na+-dependent. A model is proposed for the role of NaCl in the metabolism of reduced sulfur compounds in A. thiooxidans strain SH.

AB - The effect of NaCl and the pathways of the oxidation of reduced inorganic sulfur compounds were studied using resting cells and cell-free extracts of Acidithiobacillus thiooxidans strain SH. This isolate specifically requires NaCl for growth. The oxidation of sulfur and sulfite by resting cells was strongly inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. Carbonylcyanide m-chlorophenyl-hydrazone and monensin were also relatively strong inhibitors. Thiosulfate-oxidizing activity was not inhibited by these uncouplers. Valinomycin did not inhibit the oxidation of sulfur compounds. NaCl stimulated the sulfur- and sulfite-oxidizing activities in resting cells but not in cell-free extracts. The tetrathionate-oxidizing activity in resting cells was slightly stimulated by NaCl, whereas it did not influence the thiosulfate-oxidizing activity. Sulfide oxidation was biphasic, suggesting the formation of intermediate sulfur. The initial phase of sulfide oxidation was not affected by NaCl, whereas the subsequent oxidation of sulfur in the second phase was Na+-dependent. A model is proposed for the role of NaCl in the metabolism of reduced sulfur compounds in A. thiooxidans strain SH.

KW - Acidithiobacillus thiooxidans

KW - Acidophile

KW - Chemolithotroph

KW - Halophile

KW - Sulfur-oxidizing bacterium

UR - http://www.scopus.com/inward/record.url?scp=14644437791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644437791&partnerID=8YFLogxK

U2 - 10.1007/s00792-004-0420-5

DO - 10.1007/s00792-004-0420-5

M3 - Article

C2 - 15375674

AN - SCOPUS:14644437791

VL - 9

SP - 45

EP - 51

JO - Extremophiles : life under extreme conditions

JF - Extremophiles : life under extreme conditions

SN - 1431-0651

IS - 1

ER -