Effects of increasing concentration of salts on the expression of omp40 gene encoding a major porin of Acidithiobacillus ferrooxidans

Mohammed A. Manchur, Tadayoshi Kanao, Tsuyoshi Sugio, Kazuo Kamimura

Research output: Contribution to journalArticle

Abstract

Effects of increasing concentration of NaCl, KCl and Na2SO4 on the growth of Acidithiobacillus ferrooxidans cells and on the expression of omp40 gene, encoding one of the major outer membrane proteins of A. ferrooxidans, were examined to obtain information on the response of A. ferrooxidans NASF-1 cells to environmental changes and to improve the bioleaching system operated in salt-containing environments. Although NaCl and KCl inhibited growth of A. ferrooxidans NASF-1 cells at the concentration of 0.3 M, A. ferrooxidans NASF-1 could grow in medium supplemented with 0.5 M Na2SO4. An analysis of outer membrane proteins by a polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate revealed that the relative amount of Omp40 was increased when cells were grown in Fe2+-medium supplemented with NaCl. Northern blot hybridization analyses also revealed that the transcription of omp40 gene was stimulated in cells incubated in medium supplemented with NaCl and KCl, but not with Na2SO4.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalJournal of Biological Sciences
Volume8
Issue number1
Publication statusPublished - Jan 1 2008

Fingerprint

Acidithiobacillus
Porins
Salts
Gene Expression
Growth
Northern Blotting
Polyacrylamide Gel Electrophoresis
Membrane Proteins
Sodium
Genes

Keywords

  • Acidophile
  • Iron-oxidizing bacterium
  • Osmotic pressure
  • Outer membrane protein
  • Western blotting analysis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

Effects of increasing concentration of salts on the expression of omp40 gene encoding a major porin of Acidithiobacillus ferrooxidans. / Manchur, Mohammed A.; Kanao, Tadayoshi; Sugio, Tsuyoshi; Kamimura, Kazuo.

In: Journal of Biological Sciences, Vol. 8, No. 1, 01.01.2008, p. 125-130.

Research output: Contribution to journalArticle

@article{654dca0be6244b7b92af361638bfa02c,
title = "Effects of increasing concentration of salts on the expression of omp40 gene encoding a major porin of Acidithiobacillus ferrooxidans",
abstract = "Effects of increasing concentration of NaCl, KCl and Na2SO4 on the growth of Acidithiobacillus ferrooxidans cells and on the expression of omp40 gene, encoding one of the major outer membrane proteins of A. ferrooxidans, were examined to obtain information on the response of A. ferrooxidans NASF-1 cells to environmental changes and to improve the bioleaching system operated in salt-containing environments. Although NaCl and KCl inhibited growth of A. ferrooxidans NASF-1 cells at the concentration of 0.3 M, A. ferrooxidans NASF-1 could grow in medium supplemented with 0.5 M Na2SO4. An analysis of outer membrane proteins by a polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate revealed that the relative amount of Omp40 was increased when cells were grown in Fe2+-medium supplemented with NaCl. Northern blot hybridization analyses also revealed that the transcription of omp40 gene was stimulated in cells incubated in medium supplemented with NaCl and KCl, but not with Na2SO4.",
keywords = "Acidophile, Iron-oxidizing bacterium, Osmotic pressure, Outer membrane protein, Western blotting analysis",
author = "Manchur, {Mohammed A.} and Tadayoshi Kanao and Tsuyoshi Sugio and Kazuo Kamimura",
year = "2008",
month = "1",
day = "1",
language = "English",
volume = "8",
pages = "125--130",
journal = "Journal of Biological Sciences",
issn = "1727-3048",
publisher = "Asian Network for Scientific Information",
number = "1",

}

TY - JOUR

T1 - Effects of increasing concentration of salts on the expression of omp40 gene encoding a major porin of Acidithiobacillus ferrooxidans

AU - Manchur, Mohammed A.

AU - Kanao, Tadayoshi

AU - Sugio, Tsuyoshi

AU - Kamimura, Kazuo

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Effects of increasing concentration of NaCl, KCl and Na2SO4 on the growth of Acidithiobacillus ferrooxidans cells and on the expression of omp40 gene, encoding one of the major outer membrane proteins of A. ferrooxidans, were examined to obtain information on the response of A. ferrooxidans NASF-1 cells to environmental changes and to improve the bioleaching system operated in salt-containing environments. Although NaCl and KCl inhibited growth of A. ferrooxidans NASF-1 cells at the concentration of 0.3 M, A. ferrooxidans NASF-1 could grow in medium supplemented with 0.5 M Na2SO4. An analysis of outer membrane proteins by a polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate revealed that the relative amount of Omp40 was increased when cells were grown in Fe2+-medium supplemented with NaCl. Northern blot hybridization analyses also revealed that the transcription of omp40 gene was stimulated in cells incubated in medium supplemented with NaCl and KCl, but not with Na2SO4.

AB - Effects of increasing concentration of NaCl, KCl and Na2SO4 on the growth of Acidithiobacillus ferrooxidans cells and on the expression of omp40 gene, encoding one of the major outer membrane proteins of A. ferrooxidans, were examined to obtain information on the response of A. ferrooxidans NASF-1 cells to environmental changes and to improve the bioleaching system operated in salt-containing environments. Although NaCl and KCl inhibited growth of A. ferrooxidans NASF-1 cells at the concentration of 0.3 M, A. ferrooxidans NASF-1 could grow in medium supplemented with 0.5 M Na2SO4. An analysis of outer membrane proteins by a polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate revealed that the relative amount of Omp40 was increased when cells were grown in Fe2+-medium supplemented with NaCl. Northern blot hybridization analyses also revealed that the transcription of omp40 gene was stimulated in cells incubated in medium supplemented with NaCl and KCl, but not with Na2SO4.

KW - Acidophile

KW - Iron-oxidizing bacterium

KW - Osmotic pressure

KW - Outer membrane protein

KW - Western blotting analysis

UR - http://www.scopus.com/inward/record.url?scp=42449130937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42449130937&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:42449130937

VL - 8

SP - 125

EP - 130

JO - Journal of Biological Sciences

JF - Journal of Biological Sciences

SN - 1727-3048

IS - 1

ER -