Effects of increasing concentration of salts on the expression of omp40 gene encoding a major porin of Acidithiobacillus ferrooxidans

Mohammed A. Manchur, Tadayoshi Kanao, Tsuyoshi Sugio, Kazuo Kamimura

Research output: Contribution to journalArticle

Abstract

Effects of increasing concentration of NaCl, KCl and Na2SO4 on the growth of Acidithiobacillus ferrooxidans cells and on the expression of omp40 gene, encoding one of the major outer membrane proteins of A. ferrooxidans, were examined to obtain information on the response of A. ferrooxidans NASF-1 cells to environmental changes and to improve the bioleaching system operated in salt-containing environments. Although NaCl and KCl inhibited growth of A. ferrooxidans NASF-1 cells at the concentration of 0.3 M, A. ferrooxidans NASF-1 could grow in medium supplemented with 0.5 M Na2SO4. An analysis of outer membrane proteins by a polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate revealed that the relative amount of Omp40 was increased when cells were grown in Fe2+-medium supplemented with NaCl. Northern blot hybridization analyses also revealed that the transcription of omp40 gene was stimulated in cells incubated in medium supplemented with NaCl and KCl, but not with Na2SO4.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalJournal of Biological Sciences
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1 2008

Keywords

  • Acidophile
  • Iron-oxidizing bacterium
  • Osmotic pressure
  • Outer membrane protein
  • Western blotting analysis

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

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