Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots

Minobu Kasai, Yoko Yamamoto, Masayoshi Maeshima, Hideaki Matsumoto

Research output: Contribution to journal › Article

38 Citations (Scopus)

Abstract

We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO--inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.

Original languageEnglish
Pages (from-to)1107-1115
Number of pages9
JournalPlant and Cell Physiology
Volume34
Issue number7
Publication statusPublished - Oct 1993

Fingerprint

Tonoplast
Proton Pumps
Cytokinins
Cytokinin
Abscisic Acid
abscisic acid
proton pump
Barley
tonoplast
Vesicles
Hordeum
vesicle
barley
cytokinins
Pump
pump
Membrane
Roots
Pumps
membrane

Keywords

  • Abscisic acid
  • Cytokinin
  • H+ pumps
  • Tonoplast

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots. / Kasai, Minobu; Yamamoto, Yoko; Maeshima, Masayoshi; Matsumoto, Hideaki.

In: Plant and Cell Physiology, Vol. 34, No. 7, 10.1993, p. 1107-1115.

Research output: Contribution to journal › Article

Kasai, M, Yamamoto, Y, Maeshima, M & Matsumoto, H 1993, 'Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots', Plant and Cell Physiology, vol. 34, no. 7, pp. 1107-1115.
Kasai M, Yamamoto Y, Maeshima M, Matsumoto H. Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots. Plant and Cell Physiology. 1993 Oct;34(7):1107-1115.
Kasai, Minobu ; Yamamoto, Yoko ; Maeshima, Masayoshi ; Matsumoto, Hideaki. / Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots. In: Plant and Cell Physiology. 1993 ; Vol. 34, No. 7. pp. 1107-1115.
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AU - Matsumoto, Hideaki

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N2 - We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO--inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.

AB - We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO--inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.

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