Abstract
We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO--inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.
| Original language | English |
|---|---|
| Pages (from-to) | 1107-1115 |
| Number of pages | 9 |
| Journal | Plant and Cell Physiology |
| Volume | 34 |
| Issue number | 7 |
| Publication status | Published - Oct 1993 |
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Keywords
- Abscisic acid
- Cytokinin
- H+ pumps
- Tonoplast
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots. / Kasai, Minobu; Yamamoto, Yoko; Maeshima, Masayoshi; Matsumoto, Hideaki.
In: Plant and Cell Physiology, Vol. 34, No. 7, 10.1993, p. 1107-1115.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplast-enriched membrane vesicles prepared from barley roots
AU - Kasai, Minobu
AU - Yamamoto, Yoko
AU - Maeshima, Masayoshi
AU - Matsumoto, Hideaki
PY - 1993/10
Y1 - 1993/10
N2 - We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO--inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.
AB - We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO--inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.
KW - Abscisic acid
KW - Cytokinin
KW - H+ pumps
KW - Tonoplast
UR - http://www.scopus.com/inward/record.url?scp=0027138431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027138431&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0027138431
VL - 34
SP - 1107
EP - 1115
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -