Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Mehmet Gunduz, Andrea Rodriguez, Rosario S. Rivera, Racquel Z. Legeros, Miho Inoue, Noriyuki Nagai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume75
Issue number1
DOIs
Publication statusPublished - Oct 1 2005

Fingerprint

Bone
Proteins
Biocompatible Materials
Biocompatibility
Alkaline Phosphatase
recombinant human bone morphogenetic protein-2
atelocollagen
Phosphatases
Collagen
Biomaterials

Keywords

  • Alkaline phosphatase
  • Bone morphogenetic protein (BMP)
  • Collagen type 1
  • Immobilization
  • ST2 cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells. / Tsujigiwa, Hidetsugu; Nagatsuka, Hitoshi; Gunduz, Mehmet; Rodriguez, Andrea; Rivera, Rosario S.; Legeros, Racquel Z.; Inoue, Miho; Nagai, Noriyuki.

In: Journal of Biomedical Materials Research - Part A, Vol. 75, No. 1, 01.10.2005, p. 210-215.

Research output: Contribution to journalArticle

Tsujigiwa, Hidetsugu ; Nagatsuka, Hitoshi ; Gunduz, Mehmet ; Rodriguez, Andrea ; Rivera, Rosario S. ; Legeros, Racquel Z. ; Inoue, Miho ; Nagai, Noriyuki. / Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells. In: Journal of Biomedical Materials Research - Part A. 2005 ; Vol. 75, No. 1. pp. 210-215.
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