Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Mehmet Gunduz, Andrea Rodriguez, Rosario S. Rivera, Racquel Z. Legeros, Miho Inoue, Noriyuki Nagai

Research output: Contribution to journalArticle

18 Citations (Scopus)


The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Issue number1
Publication statusPublished - Oct 1 2005



  • Alkaline phosphatase
  • Bone morphogenetic protein (BMP)
  • Collagen type 1
  • Immobilization
  • ST2 cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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