Abstract
The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity.
| Original language | English |
|---|---|
| Pages (from-to) | 210-215 |
| Number of pages | 6 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 75 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 1 2005 |
Keywords
- Alkaline phosphatase
- Bone morphogenetic protein (BMP)
- Collagen type 1
- Immobilization
- ST2 cells
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys