Effects of Immobilized Recombinant Human BMP-2/Collagen on Cellular Activity of Osteoblast Like Cells

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Mehmet Gunduz, Masao Yamada, Noriyuki Nagai, Jin Tan, Katsunori Kanata

Research output: Contribution to journalArticle

Abstract

The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization. (2) Studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated in augmenting cellular activity.

Original languageEnglish
Pages (from-to)126-127
Number of pages2
JournalJournal of Hard Tissue Biology
Volume14
Issue number2
DOIs
Publication statusPublished - 2005

Keywords

  • alkaline phosphatase
  • BMP
  • collagen Type 1
  • immobilization
  • ST2cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

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