Effects of Immobilized Recombinant Human BMP-2/Collagen on Cellular Activity of Osteoblast Like Cells

Hidetsugu Tsujigiwa; Hitoshi Nagatsuka; Mehmet Gunduz; Masao Yamada; Noriyuki Nagai; Jin Tan; Katsunori Kanata

doi:10.2485/jhtb.14.126

Effects of Immobilized Recombinant Human BMP-2/Collagen on Cellular Activity of Osteoblast Like Cells

Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Mehmet Gunduz, Masao Yamada, Noriyuki Nagai, Jin Tan, Katsunori Kanata

Research output: Contribution to journal › Article › peer-review

Abstract

The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization. (2) Studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated in augmenting cellular activity.

Original language	English
Pages (from-to)	126-127
Number of pages	2
Journal	Journal of Hard Tissue Biology
Volume	14
Issue number	2
DOIs	https://doi.org/10.2485/jhtb.14.126
Publication status	Published - 2005

Keywords

alkaline phosphatase
BMP
collagen Type 1
immobilization
ST2cells

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry
Biomaterials
Orthopedics and Sports Medicine
Dentistry(all)
Cell Biology

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10.2485/jhtb.14.126

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title = "Effects of Immobilized Recombinant Human BMP-2/Collagen on Cellular Activity of Osteoblast Like Cells",

abstract = "The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization. (2) Studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated in augmenting cellular activity.",

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AU - Tsujigiwa, Hidetsugu

AU - Nagatsuka, Hitoshi

AU - Gunduz, Mehmet

AU - Yamada, Masao

AU - Nagai, Noriyuki

AU - Tan, Jin

AU - Kanata, Katsunori

PY - 2005

Y1 - 2005

N2 - The use of rhBMP-2 to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features and biocompatibility, is known to be an effective carrier for rhBMP-2. However, conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization. (2) Studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated in augmenting cellular activity.

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Effects of Immobilized Recombinant Human BMP-2/Collagen on Cellular Activity of Osteoblast Like Cells

Abstract

Keywords

ASJC Scopus subject areas

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