Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales

Kei ichiro Kitamura, Tadashi Andoh, Wakana Okesaku, Yuya Tazaki, Kazuhiro Ogai, Kayo Sugitani, Isao Kobayashi, Nobuo Suzuki, Wenxi Chen, Mika Ikegame, Atsuhiko Hattori

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Increased risk of fracture associated with type 2 diabetes has been a topic of recent concern. Fracture risk is related to a decrease in bone strength, which can be affected by bone metabolism and the quality of the bone. To investigate the cause of the increased fracture rate in patients with diabetes through analyses of bone metabolism and bone matrix protein properties, we used goldfish scales as a bone model for hyperglycemia. Using the scales of seven alloxan-treated and seven vehicle-treated control goldfish, we assessed bone metabolism by analyzing the activity of marker enzymes and mRNA expression of marker genes, and we measured the change in molecular weight of scale matrix proteins with SDS-PAGE. After only a 2-week exposure to hyperglycemia, the molecular weight of α- and β-fractions of bone matrix collagen proteins changed incrementally in the regenerating scales of hyperglycemic goldfish compared with those of euglycemic goldfish. In addition, the relative ratio of the γ-fraction significantly increased, and a δ-fraction appeared after adding glyceraldehyde—a candidate for the formation of advanced glycation end products in diabetes—to isolated type 1 collagen in vitro. The enzymatic activity and mRNA expression of osteoblast and osteoclast markers were not significantly different between hyperglycemic and euglycemic goldfish scales. These results indicate that hyperglycemia is likely to affect bone quality through glycation of matrix collagen from an early stage of hyperglycemia. Therefore, non-enzymatic glycation of collagen fibers in bone matrix may lead to the deterioration of bone quality from the onset of diabetes.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume203
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Goldfish
Bone Matrix
Metabolism
Hyperglycemia
Bone
Bone and Bones
Collagen
Medical problems
Molecular Weight
Messenger RNA
Alloxan
Advanced Glycosylation End Products
Proteins
Molecular weight
Osteoclasts
Collagen Type I
Osteoblasts
Type 2 Diabetes Mellitus
Polyacrylamide Gel Electrophoresis
Gene Expression

Keywords

  • Alloxan
  • Bone metabolism
  • Bone model
  • Diabetes
  • Fish scales
  • Glycation of matrix collagen
  • Hyperglycemia
  • Increased fracture rate
  • Type 1 collagen

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology

Cite this

Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales. / Kitamura, Kei ichiro; Andoh, Tadashi; Okesaku, Wakana; Tazaki, Yuya; Ogai, Kazuhiro; Sugitani, Kayo; Kobayashi, Isao; Suzuki, Nobuo; Chen, Wenxi; Ikegame, Mika; Hattori, Atsuhiko.

In: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, Vol. 203, 01.01.2017, p. 152-158.

Research output: Contribution to journalArticle

Kitamura, KI, Andoh, T, Okesaku, W, Tazaki, Y, Ogai, K, Sugitani, K, Kobayashi, I, Suzuki, N, Chen, W, Ikegame, M & Hattori, A 2017, 'Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales', Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, vol. 203, pp. 152-158. https://doi.org/10.1016/j.cbpa.2016.09.010
Kitamura, Kei ichiro ; Andoh, Tadashi ; Okesaku, Wakana ; Tazaki, Yuya ; Ogai, Kazuhiro ; Sugitani, Kayo ; Kobayashi, Isao ; Suzuki, Nobuo ; Chen, Wenxi ; Ikegame, Mika ; Hattori, Atsuhiko. / Effects of hyperglycemia on bone metabolism and bone matrix in goldfish scales. In: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology. 2017 ; Vol. 203. pp. 152-158.
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