Effects of human serum albumin on absorption and fluorescence spectra of dyes related to Chrome Azurol S.

Yutaka Saito, Masaki Mifune, Junichi Odo, Kumiko Iwasaki, Yoshimasa Tanaka, Hideki Okuda

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3 Citations (Scopus)

Abstract

In order to reveal a functional group required to form an analytically valuable complex such as chrome Azurol S (CAS)-human serum albumin (HSA) complex, the absorption and fluorescence spectra of ten dyes related to CAS were examined in the presence of HSA. A red shift similar to that caused by HSA in the case of CAS was observed in the absorption spectrum only for the dyes containing carboxyl group. HSA also caused increase of fluorescence of these dyes, whereas the dyes free from carboxyl group exhibited weak or no fluorescence even in the presence of HSA. In particular, the increase of fluorescence intensity of Eriochrome Cyanine R (ECR) was found to be larger than that of CAS, and ECR may be superior to CAS for the determination of HSA. The increase of fluorescence may be attributed to ionization of carboxyl group and/or wear of planarity of the dye by binding to HSA. The results indicate that carboxyl group is essential to form an analytically valuable dye-HSA complex in contrast with the case of Bromcresol Green-HSA complex.

Original languageEnglish
Pages (from-to)987-990
Number of pages4
JournalBunseki Kagaku
Volume35
Issue number12
DOIs
Publication statusPublished - 1986
Externally publishedYes

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Keywords

  • absorption spectra
  • determination of human serum albumin
  • dye-binding method
  • dyes interation
  • dyes related to Chrome Azurol S
  • fluorescence spectra
  • human serum albumin

ASJC Scopus subject areas

  • Analytical Chemistry

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