Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts

Takashi Oono, Yoshinori Shirafuji, Wook Kang Huh, Hisanori Akiyama, Keiji Iwatsuki

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31 Citations (Scopus)


Human neutrophil peptide-1 (HNP-1), a defensin with antimicrobial properties, is also thought to promote wound healing. To elucidate the mechanism by which wound healing is facilitated by this factor, we investigated the effect of HNP-1 on the expression of interstitial collagenase (matrix metalloproteinase 1, MMP-1), collagen types I and III, and tissue inhibitor of metalloproteinase 1 (TIMP-1) by cultured fibroblasts by means of RT-PCR and ELISA. Our results showed that synthetic HNP-1 increased the expression of proα1(I) collagen mRNA and protein. In contrast, the expression of MMP-1 was decreased at both the mRNA and protein levels. Our observations suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition and by controlling its degradation.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalArchives of Dermatological Research
Issue number4
Publication statusPublished - 2002



  • Collagen
  • Collagenase
  • Defensin
  • Human neutrophil peptide
  • Wound healing

ASJC Scopus subject areas

  • Dermatology

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