Effects of Fluid Bolus Therapy on Renal Perfusion, Oxygenation, and Function in Early Experimental Septic Kidney Injury

Yugeesh R. Lankadeva, Junko Kosaka, Naoya Iguchi, Roger G. Evans, Lindsea C. Booth, Rinaldo Bellomo, Clive N. May

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: To examine the effects of fluid bolus therapy on systemic hemodynamics, renal blood flow, intrarenal perfusion and oxygenation, Po 2 , renal function, and fluid balance in experimental early septic acute kidney injury. Design: Interventional study. Setting: Research institute. Subjects: Adult Merino ewes. Interventions: Implantation of flow probes on the pulmonary and renal arteries and laser Doppler oxygen-sensing probes in the renal cortex, medulla, and within a bladder catheter in sheep. Infusion of Escherichia coli to induce septic acute kidney injury (n = 8). After 24, 25, and 26 hours of sepsis, fluid bolus therapy (500 mL of Hartmann's solution over 15 min) was administered. Measurements and Main Results: In conscious sheep, infusion of Escherichia coli decreased creatinine clearance and increased plasma creatinine, renal blood flow (+46% ± 6%) and cortical perfusion (+25% ± 4%), but medullary perfusion (-48% ± 5%), medullary Po 2 (-56% ± 4%), and urinary Po 2 (-54% ± 3%) decreased (p < 0.01). The first fluid bolus therapy increased blood pressure (+6% ± 1%), central venous pressure (+245% ± 65%), cardiac output (+11% ± 2%), medullary Po 2 (+280% ± 90%), urinary Po 2 (+164% ± 80%), and creatinine clearance (+120% ± 65%) at 30 minutes. The following two boluses had no beneficial effects on creatinine clearance. The improvement in medullary oxygenation dissipated following the third fluid bolus therapy. Study animals retained 69% of the total volume and 80% of sodium infused. Throughout the study, urinary Po 2 correlated significantly with medullary Po 2 . Conclusions: In early experimental septic acute kidney injury, fluid bolus therapy transiently improved renal function and medullary Po 2 , as also reflected by increased urinary Po 2. These initial effects of fluid bolus therapy dissipated within 4 hours, despite two additional fluid boluses, and resulted in significant volume retention.

Original languageEnglish
Pages (from-to)E36-E43
JournalCritical care medicine
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Fingerprint

Fluid Therapy
Perfusion
Kidney
Creatinine
Wounds and Injuries
Acute Kidney Injury
Renal Circulation
Sheep
Escherichia coli
Central Venous Pressure
Water-Electrolyte Balance
Renal Artery
Cardiac Output
Pulmonary Artery
Sepsis
Urinary Bladder
Lasers
Catheters
Hemodynamics
Sodium

Keywords

  • acute kidney injury
  • fluid bolus therapy
  • hypoxia
  • sepsis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Effects of Fluid Bolus Therapy on Renal Perfusion, Oxygenation, and Function in Early Experimental Septic Kidney Injury. / Lankadeva, Yugeesh R.; Kosaka, Junko; Iguchi, Naoya; Evans, Roger G.; Booth, Lindsea C.; Bellomo, Rinaldo; May, Clive N.

In: Critical care medicine, Vol. 47, No. 1, 01.01.2019, p. E36-E43.

Research output: Contribution to journalArticle

Lankadeva, Yugeesh R. ; Kosaka, Junko ; Iguchi, Naoya ; Evans, Roger G. ; Booth, Lindsea C. ; Bellomo, Rinaldo ; May, Clive N. / Effects of Fluid Bolus Therapy on Renal Perfusion, Oxygenation, and Function in Early Experimental Septic Kidney Injury. In: Critical care medicine. 2019 ; Vol. 47, No. 1. pp. E36-E43.
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