Effects of extrinsic proteins on the protein conformation of the oxygen-evolving center in cyanobacterial photosystem II as revealed by fourier transform infrared spectroscopy

Ryo Nagao, Tatsuya Tomo, Takumi Noguchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Extrinsic proteins of photosystem II (PSII) play an important role in optimizing oxygen-evolving reactions in all oxyphototrophs. The currently available crystal structures of cyanobacterial PSII core complexes show the binding structures of the extrinsic proteins, PsbO, PsbV, and PsbU; however, how the individual extrinsic proteins affect the structure and the function of the oxygen-evolving center (OEC) in cyanobacterial PSII remains unknown. In this study, we have investigated the effects of the binding of the extrinsic proteins on the protein conformation of the OEC in PSII core complexes from the thermophilic cyanobacterium Thermosynechococcus elongatus, using light-induced Fourier transform infrared (FTIR) difference spectroscopy. Upon removal of the three extrinsic proteins, an S2-minus-S1 FTIR difference spectrum measured in the presence of a high CaCl2 concentration showed a drastic change in amide I bands, reflecting perturbation of the secondary structures of polypeptides, whereas the overall spectral intensity was lost at a low CaCl2 concentration, indicative of inactivation of the Mn4CaO5 cluster. The amide I features as well as the overall intensity were recovered mainly by binding of PsbO, while complete amide I recovery was achieved by further binding of PsbV and PsbU. We thus concluded that PsbO, together with smaller contributions of PsbV and PsbU, plays a role in the maintenance of the proper protein conformation of the OEC in cyanobacterial PSII, which provides the stability of the Mn4CaO5 cluster via the enhanced retention capability of Ca2+ and Cl- ions.

Original languageEnglish
Pages (from-to)2022-2031
Number of pages10
JournalBiochemistry
Volume54
Issue number11
DOIs
Publication statusPublished - Mar 24 2015
Externally publishedYes

Fingerprint

Protein Conformation
Photosystem II Protein Complex
Fourier Transform Infrared Spectroscopy
Conformations
Oxygen
Amides
Proteins
Fourier transforms
Cyanobacteria
Fourier Analysis
Infrared radiation
Carrier Proteins
Maintenance
Ions
Light
Peptides
Crystal structure
Spectroscopy
Recovery

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Effects of extrinsic proteins on the protein conformation of the oxygen-evolving center in cyanobacterial photosystem II as revealed by fourier transform infrared spectroscopy. / Nagao, Ryo; Tomo, Tatsuya; Noguchi, Takumi.

In: Biochemistry, Vol. 54, No. 11, 24.03.2015, p. 2022-2031.

Research output: Contribution to journalArticle

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