Effects of exogenous application of proline and betaine on the growth of tobacco cultured cells under saline conditions

Eiji Okuma, Yuki Murakami, Yasuaki Shimoishi, Mikiro Tada, Yoshiyuki Murata

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The effects of the application of exogenous proline and betaine on the growth of tobacco cultured cells subjected to salt stress were investigated. Both proline and betaine mitigated the inhibition of growth of tobacco cells under saline conditions, but the harmful effect of salinity was less reduced by betaine than by proline. The amount of intracellular betaine in tobacco cells cultured in the NaCl medium supplemented with 20 mM betaine was larger than that of intracellular proline in the NaCl medium with 20 mM proline. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) experiments showed that proline displayed an antioxidant activity and that the antioxidant activity of betaine was not detectable. The malondialdehyde (MDA) assay demonstrated that exogenous proline but not betaine decreased the amount of MDA in salt-stressed tobacco cells. These results suggest that the difference in the mitigation effects between proline and betaine may be responsible for the difference in the antioxidant activity.

Original languageEnglish
Pages (from-to)1301-1305
Number of pages5
JournalSoil Science and Plant Nutrition
Volume50
Issue number8
Publication statusPublished - Dec 2004

Fingerprint

betaine
tobacco
cultured cells
proline
antioxidant
salt
antioxidant activity
malondialdehyde
mitigation
assay
salinity
effect
growth retardation
salt stress
experiment
cells
salts
assays

Keywords

  • Antioxidant activity
  • Betaine
  • Nicotiana tabacum
  • Proline
  • Salt stress

ASJC Scopus subject areas

  • Soil Science
  • Agronomy and Crop Science

Cite this

Effects of exogenous application of proline and betaine on the growth of tobacco cultured cells under saline conditions. / Okuma, Eiji; Murakami, Yuki; Shimoishi, Yasuaki; Tada, Mikiro; Murata, Yoshiyuki.

In: Soil Science and Plant Nutrition, Vol. 50, No. 8, 12.2004, p. 1301-1305.

Research output: Contribution to journalArticle

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