Effects of exercise training on gingival oxidative stress in obese rats

Tetsuji Azuma, Takaaki Tomofuji, Yasumasa Endo, Naofumi Tamaki, Daisuke Ekuni, Koichiro Irie, Kenta Kasuyama, Tomo Kato, Manabu Morita

Research output: Contribution to journalArticle

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Abstract

Objective: The purpose of the present study was to investigate the effects of exercise training on serum reactive oxygen species (ROS) level and gingival oxidative stress in obese rats fed a high-fat diet. Design: Rats were divided into three groups (n = 14/group): one control group (fed a regular diet) and two experimental groups (fed a high-fat diet with and without exercise training [treadmill: 5 days/week]). The rats were sacrificed at 4 or 8 weeks. The level of serum reactive oxidative metabolites (ROM) was measured as an indicator of circulating ROS. The level of 8-hydroxydeoxyguanosine (8-OHdG) and reduced-form glutathione (GSH)/oxidised-form glutathione (GSSG) ratio were determined to evaluate gingival oxidative stress. Results: The obese rats fed a high-fat diet without exercise training showed higher serum ROM levels [Carratelli Units (CARR U)] (mean ± SD; 413 ± 64) than the control (333 ± 12) at 4 weeks (p = 0.023). Such a condition resulted in higher 8-OHdG levels (ng/mg mtDNA) (0.97 ± 0.18) (p <0.05) and a lower GSH/GSSG ratio (17.0 ± 3.1) (p <0.05) in gingival tissues, compared to the control (0.55 ± 0.13 for 8-OHdG and 23.6 ± 5.8 for GSH/GSSG ratio) at 8 weeks. In addition, the obese rats fed a high-fat diet with exercise training showed lower serum ROM (623 ± 103) (p <0.001) and gingival 8-OHdG levels (0.69 ± 0.17) (p = 0.012) than those without exercise training (1105 ± 95 for ROM and 0.55 ± 0.13 for 8-OHdG) at 8 weeks. Conclusions: Obesity prevention by exercise training may effectively suppress gingival oxidative stress by decreasing serum ROS in rats.

Original languageEnglish
Pages (from-to)768-774
Number of pages7
JournalArchives of Oral Biology
Volume56
Issue number8
DOIs
Publication statusPublished - Aug 2011

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Oxidative Stress
High Fat Diet
Exercise
Glutathione Disulfide
Reactive Oxygen Species
Serum
Mitochondrial DNA
Glutathione
Obesity
Diet
Control Groups

Keywords

  • Exercise training
  • Obesity
  • Oxidative stress
  • Periodontal diseases

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Effects of exercise training on gingival oxidative stress in obese rats. / Azuma, Tetsuji; Tomofuji, Takaaki; Endo, Yasumasa; Tamaki, Naofumi; Ekuni, Daisuke; Irie, Koichiro; Kasuyama, Kenta; Kato, Tomo; Morita, Manabu.

In: Archives of Oral Biology, Vol. 56, No. 8, 08.2011, p. 768-774.

Research output: Contribution to journalArticle

Azuma, T, Tomofuji, T, Endo, Y, Tamaki, N, Ekuni, D, Irie, K, Kasuyama, K, Kato, T & Morita, M 2011, 'Effects of exercise training on gingival oxidative stress in obese rats', Archives of Oral Biology, vol. 56, no. 8, pp. 768-774. https://doi.org/10.1016/j.archoralbio.2011.01.008
Azuma, Tetsuji ; Tomofuji, Takaaki ; Endo, Yasumasa ; Tamaki, Naofumi ; Ekuni, Daisuke ; Irie, Koichiro ; Kasuyama, Kenta ; Kato, Tomo ; Morita, Manabu. / Effects of exercise training on gingival oxidative stress in obese rats. In: Archives of Oral Biology. 2011 ; Vol. 56, No. 8. pp. 768-774.
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AU - Ekuni, Daisuke

AU - Irie, Koichiro

AU - Kasuyama, Kenta

AU - Kato, Tomo

AU - Morita, Manabu

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