Effects of diethyldithiocarbamate on activating mechanisms of neutrophils

Y. Izumi, Keiki Ogino, T. Murata, H. Kobayashi, T. Hobara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effects of diethyldithiocarbamate on superoxide release by rat neurophils were investigated in a chemiluminescence study. Diethyldithiocarbamate augmented lucigenin-dependent chemiluminescence in a concentration-dependent manner and inhibited luminol-dependent chemiluminescence at concentrations of 0.1-1 μM. In contrast, after the addition of 0.1 mM diethyldithiocarbamate, the chemiluminescence was markedly enhanced. Diethyldithiocarbamate inhibited both the myeloperoxidase activity of neutrophils and the chemiluminescence generated in a cell-free horseradish peroxidase/H2O2 and H2O2/HOCl system. The increase in lucigenin-dependent chemiluminescence brought about by diethyldithiocarbamate was inhibited by H-7, ML-7, W-7, EGTA and pertussis toxin. These results suggest that diethyldithiocarbamate may stimulate O2- production by a guanosine 5'-triphosphate protein-mediated and Ca2+-dependent process and that the increase in O2- release by neutrophils may be dependent not only on the direct stimulation of the signal transduction pathway but also on the increase in O2- by reducing the effect of the hydroperoxide (H2O2)-myeloperoxidase system.

Original languageEnglish
Pages (from-to)280-286
Number of pages7
JournalPharmacology and Toxicology
Volume74
Issue number4-5
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Ditiocarb
Chemiluminescence
Luminescence
Neutrophils
Peroxidase
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Luminol
Signal transduction
Guanosine
Egtazic Acid
Pertussis Toxin
Horseradish Peroxidase
Guanosine Triphosphate
Superoxides
Hydrogen Peroxide
Rats
Signal Transduction
Proteins

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Izumi, Y., Ogino, K., Murata, T., Kobayashi, H., & Hobara, T. (1994). Effects of diethyldithiocarbamate on activating mechanisms of neutrophils. Pharmacology and Toxicology, 74(4-5), 280-286.

Effects of diethyldithiocarbamate on activating mechanisms of neutrophils. / Izumi, Y.; Ogino, Keiki; Murata, T.; Kobayashi, H.; Hobara, T.

In: Pharmacology and Toxicology, Vol. 74, No. 4-5, 1994, p. 280-286.

Research output: Contribution to journalArticle

Izumi, Y, Ogino, K, Murata, T, Kobayashi, H & Hobara, T 1994, 'Effects of diethyldithiocarbamate on activating mechanisms of neutrophils', Pharmacology and Toxicology, vol. 74, no. 4-5, pp. 280-286.
Izumi Y, Ogino K, Murata T, Kobayashi H, Hobara T. Effects of diethyldithiocarbamate on activating mechanisms of neutrophils. Pharmacology and Toxicology. 1994;74(4-5):280-286.
Izumi, Y. ; Ogino, Keiki ; Murata, T. ; Kobayashi, H. ; Hobara, T. / Effects of diethyldithiocarbamate on activating mechanisms of neutrophils. In: Pharmacology and Toxicology. 1994 ; Vol. 74, No. 4-5. pp. 280-286.
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