TY - JOUR
T1 - Effects of Diethyldithiocarbamate on Activating Mechanisms of Neutrophils
AU - Izumi, Yukie
AU - Ogino, Keiki
AU - Murata, Tomoko
AU - Kobayashi, Haruo
AU - Hobara, Tatsuya
PY - 1994/6
Y1 - 1994/6
N2 - The effects of diethyldithiocarbamate on superoxide release by rat neurophils were investigated in a chemiluminescence study. Diethyldithiocarbamate augmented lucigenin‐dependent chemiluminescence in a concentration‐dependent manner and inhibited luminol‐dependent chemiluminescence at concentrations of 0.1‐1 μM. In contrast, after the addition of 0.1 mM diethyldithiocarbamate, the chemiluminescence was markedly enhanced. Diethyldithiocarbamate inhibited both the myeloperoxidase activity of neutrophils and the chemiluminescence generated in a cell‐free horseradish peroxidase/H2O2 and H2O2/HOCl system. The increase in lucigenin‐dependent chemiluminescence brought about by diethyldithiocarbamate was inhibited by H‐7, ML‐7, W‐7, EGTA and pertussis toxin. These results suggest that diethyldithiocarbamate may stimulate O2− production by a guanosine 5′‐triphosphate protein‐mediated and Ca2+–dependent process and that the increase in O2− release by neutrophils may be dependent not only on the direct stimulation of the signal transduction pathway but also on the increase in O2− by reducing the effect of the hydroperoxide (H2O2)‐myeloperoxidase system. 1994 Nordic Pharmacological Society
AB - The effects of diethyldithiocarbamate on superoxide release by rat neurophils were investigated in a chemiluminescence study. Diethyldithiocarbamate augmented lucigenin‐dependent chemiluminescence in a concentration‐dependent manner and inhibited luminol‐dependent chemiluminescence at concentrations of 0.1‐1 μM. In contrast, after the addition of 0.1 mM diethyldithiocarbamate, the chemiluminescence was markedly enhanced. Diethyldithiocarbamate inhibited both the myeloperoxidase activity of neutrophils and the chemiluminescence generated in a cell‐free horseradish peroxidase/H2O2 and H2O2/HOCl system. The increase in lucigenin‐dependent chemiluminescence brought about by diethyldithiocarbamate was inhibited by H‐7, ML‐7, W‐7, EGTA and pertussis toxin. These results suggest that diethyldithiocarbamate may stimulate O2− production by a guanosine 5′‐triphosphate protein‐mediated and Ca2+–dependent process and that the increase in O2− release by neutrophils may be dependent not only on the direct stimulation of the signal transduction pathway but also on the increase in O2− by reducing the effect of the hydroperoxide (H2O2)‐myeloperoxidase system. 1994 Nordic Pharmacological Society
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U2 - 10.1111/j.1600-0773.1994.tb01112.x
DO - 10.1111/j.1600-0773.1994.tb01112.x
M3 - Article
C2 - 8090700
AN - SCOPUS:0028353067
VL - 74
SP - 280
EP - 286
JO - Basic and Clinical Pharmacology and Toxicology
JF - Basic and Clinical Pharmacology and Toxicology
SN - 1742-7835
IS - 6
ER -