Effects of Diethyldithiocarbamate on Activating Mechanisms of Neutrophils

Yukie Izumi, Keiki Ogino, Tomoko Murata, Haruo Kobayashi, Tatsuya Hobara

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The effects of diethyldithiocarbamate on superoxide release by rat neurophils were investigated in a chemiluminescence study. Diethyldithiocarbamate augmented lucigenin‐dependent chemiluminescence in a concentration‐dependent manner and inhibited luminol‐dependent chemiluminescence at concentrations of 0.1‐1 μM. In contrast, after the addition of 0.1 mM diethyldithiocarbamate, the chemiluminescence was markedly enhanced. Diethyldithiocarbamate inhibited both the myeloperoxidase activity of neutrophils and the chemiluminescence generated in a cell‐free horseradish peroxidase/H2O2 and H2O2/HOCl system. The increase in lucigenin‐dependent chemiluminescence brought about by diethyldithiocarbamate was inhibited by H‐7, ML‐7, W‐7, EGTA and pertussis toxin. These results suggest that diethyldithiocarbamate may stimulate O2 production by a guanosine 5′‐triphosphate protein‐mediated and Ca2+–dependent process and that the increase in O2 release by neutrophils may be dependent not only on the direct stimulation of the signal transduction pathway but also on the increase in O2 by reducing the effect of the hydroperoxide (H2O2)‐myeloperoxidase system. 1994 Nordic Pharmacological Society

Original languageEnglish
Pages (from-to)280-286
Number of pages7
JournalPharmacology & Toxicology
Volume74
Issue number6
DOIs
Publication statusPublished - Jun 1994

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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