Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro

Ken Sawai, Hiroaki Funahashi, Koji Niwa

Research output: Contribution to journalArticle

Abstract

The present study examined the effect of cysteine and cystine associated with cumulus cells in maturation medium on oocyte glutathione (GSH) concentration at the end of maturation culture (for 48 h) and male pronuclear (MPN) formation following in vitro fertilization of pig oocytes. When cumulus-enclosed oocytes were cultured in a serum-free medium containing 0.57 mM cysteine or cystine, their GSH concentration and incidence of MPN formation were higher than when they were cultured without cysteine and cystine. Supplementation with cysteine during the final 24 h of culture resulted in an increased GSH concentration of oocytes and a higher incidence of MPN formation, regardless of the presence of cumulus cells, than in those cultured without cysteine or cystine. The same high incidence of MPN formation was observed even when oocytes were denuded after 36 h of culture and exposed to cysteine for only 12 h. In contrast, when oocytes were denuded after 24 h of culture and exposed to cystine, neither GSH synthesis nor MPN formation was improved, but both parameters did improve when oocytes were not denuded. Exposure of cumulus-enclosed oocytes to cystine from 36 h of culture did not promote MPN formation. These results indicate that cumulus-free pig oocytes can synthesize GSH in the presence of cysteine and that the presence of cumulus cells is essential for maintaining a high concentration of GSH in oocytes in the presence of cystine.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalJournal of Reproduction and Development
Volume44
Issue number2
Publication statusPublished - 1998

Fingerprint

cystine
cysteine
oocytes
swine
cells
incidence
in vitro fertilization
glutathione
synthesis

Keywords

  • Cysteine
  • Cystine
  • Glutathione
  • Male pronuclear formation
  • Pig

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro. / Sawai, Ken; Funahashi, Hiroaki; Niwa, Koji.

In: Journal of Reproduction and Development, Vol. 44, No. 2, 1998, p. 161-168.

Research output: Contribution to journalArticle

@article{d182db51638a4c75a37ed27d989ccb4c,
title = "Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro",
abstract = "The present study examined the effect of cysteine and cystine associated with cumulus cells in maturation medium on oocyte glutathione (GSH) concentration at the end of maturation culture (for 48 h) and male pronuclear (MPN) formation following in vitro fertilization of pig oocytes. When cumulus-enclosed oocytes were cultured in a serum-free medium containing 0.57 mM cysteine or cystine, their GSH concentration and incidence of MPN formation were higher than when they were cultured without cysteine and cystine. Supplementation with cysteine during the final 24 h of culture resulted in an increased GSH concentration of oocytes and a higher incidence of MPN formation, regardless of the presence of cumulus cells, than in those cultured without cysteine or cystine. The same high incidence of MPN formation was observed even when oocytes were denuded after 36 h of culture and exposed to cysteine for only 12 h. In contrast, when oocytes were denuded after 24 h of culture and exposed to cystine, neither GSH synthesis nor MPN formation was improved, but both parameters did improve when oocytes were not denuded. Exposure of cumulus-enclosed oocytes to cystine from 36 h of culture did not promote MPN formation. These results indicate that cumulus-free pig oocytes can synthesize GSH in the presence of cysteine and that the presence of cumulus cells is essential for maintaining a high concentration of GSH in oocytes in the presence of cystine.",
keywords = "Cysteine, Cystine, Glutathione, Male pronuclear formation, Pig",
author = "Ken Sawai and Hiroaki Funahashi and Koji Niwa",
year = "1998",
language = "English",
volume = "44",
pages = "161--168",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "2",

}

TY - JOUR

T1 - Effects of cumulus cells on the ability of pig oocytes to utilize cysteine or cystine during maturation in vitro

AU - Sawai, Ken

AU - Funahashi, Hiroaki

AU - Niwa, Koji

PY - 1998

Y1 - 1998

N2 - The present study examined the effect of cysteine and cystine associated with cumulus cells in maturation medium on oocyte glutathione (GSH) concentration at the end of maturation culture (for 48 h) and male pronuclear (MPN) formation following in vitro fertilization of pig oocytes. When cumulus-enclosed oocytes were cultured in a serum-free medium containing 0.57 mM cysteine or cystine, their GSH concentration and incidence of MPN formation were higher than when they were cultured without cysteine and cystine. Supplementation with cysteine during the final 24 h of culture resulted in an increased GSH concentration of oocytes and a higher incidence of MPN formation, regardless of the presence of cumulus cells, than in those cultured without cysteine or cystine. The same high incidence of MPN formation was observed even when oocytes were denuded after 36 h of culture and exposed to cysteine for only 12 h. In contrast, when oocytes were denuded after 24 h of culture and exposed to cystine, neither GSH synthesis nor MPN formation was improved, but both parameters did improve when oocytes were not denuded. Exposure of cumulus-enclosed oocytes to cystine from 36 h of culture did not promote MPN formation. These results indicate that cumulus-free pig oocytes can synthesize GSH in the presence of cysteine and that the presence of cumulus cells is essential for maintaining a high concentration of GSH in oocytes in the presence of cystine.

AB - The present study examined the effect of cysteine and cystine associated with cumulus cells in maturation medium on oocyte glutathione (GSH) concentration at the end of maturation culture (for 48 h) and male pronuclear (MPN) formation following in vitro fertilization of pig oocytes. When cumulus-enclosed oocytes were cultured in a serum-free medium containing 0.57 mM cysteine or cystine, their GSH concentration and incidence of MPN formation were higher than when they were cultured without cysteine and cystine. Supplementation with cysteine during the final 24 h of culture resulted in an increased GSH concentration of oocytes and a higher incidence of MPN formation, regardless of the presence of cumulus cells, than in those cultured without cysteine or cystine. The same high incidence of MPN formation was observed even when oocytes were denuded after 36 h of culture and exposed to cysteine for only 12 h. In contrast, when oocytes were denuded after 24 h of culture and exposed to cystine, neither GSH synthesis nor MPN formation was improved, but both parameters did improve when oocytes were not denuded. Exposure of cumulus-enclosed oocytes to cystine from 36 h of culture did not promote MPN formation. These results indicate that cumulus-free pig oocytes can synthesize GSH in the presence of cysteine and that the presence of cumulus cells is essential for maintaining a high concentration of GSH in oocytes in the presence of cystine.

KW - Cysteine

KW - Cystine

KW - Glutathione

KW - Male pronuclear formation

KW - Pig

UR - http://www.scopus.com/inward/record.url?scp=54749129934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54749129934&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 161

EP - 168

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 2

ER -