Effects of combined β-hydroxy-β-methylbutyrate (HMB) and whey protein ingestion on symptoms of eccentric exercise-induced muscle damage

Minayuki Shirato, Yosuke Tsuchiya, Teruyuki Sato, Saki Hamano, Takeshi Gushiken, Naoto Kimura, Eisuke Ochi

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Background: The purpose of this study was to examine the effects of combined β-hydroxy-β-methylbutyrate (HMB) and whey protein ingestion on muscle strength and damage following a single bout of eccentric exercise. Methods: Eighteen untrained male subjects were assigned to HMB and Whey protein (HMB + Whey; 3 g/day HMB and 36.6 g/day whey protein, n = 6), HMB (3 g/day, n = 6), or whey protein (36.6 g/day, n = 6) groups. Ingestion commenced 7 days before non-dominant elbow flexor eccentric exercise (30 deg/sec, 6 reps × 7 sets) and continued until 4 days post-exercise. The maximal isometric strength, muscle soreness, plasma creatine kinase (CK), lactate dehydrogenase (LDH) were assessed pre-exercise, and at 1, 2, 3, and 5 days after exercise. Results: The change scores of maximal isometric strength significantly decreased at day 1, 2, and 5 in the whey protein group compared to pre value and that in HMB + Whey protein and HMB groups decreased at day 1 and 5. The muscle soreness significantly increased in the whey and HMB + Whey protein groups at day 3 compared to pre value (p <0.05). CK and LDH significantly increased (time effect: p <0.05) after exercise. However, all data were not significant difference among the groups. Conclusions: These results suggest that ingestion of combined HMB and whey protein does not have a role to inhibit muscle strength loss and soreness, and decrease in muscle damage markers after eccentric exercise in comparison with HMB and whey protein alone.

Original languageEnglish
Article number7
JournalJournal of the International Society of Sports Nutrition
Issue number1
Publication statusPublished - Feb 29 2016



  • Amino acids
  • Lengthening
  • Muscle soreness
  • Muscle strength

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

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