TY - JOUR
T1 - Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity
AU - Tsai, Y. J.
AU - Abe, H.
AU - Maruta, H.
AU - Hatano, T.
AU - Nishina, H.
AU - Sakagami, H.
AU - Okuda, T.
AU - Tanuma, S. I.
PY - 1991/12/3
Y1 - 1991/12/3
N2 - Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.
AB - Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.
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M3 - Article
C2 - 1776956
AN - SCOPUS:0025939794
VL - 24
SP - 889
EP - 897
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
SN - 1521-6543
IS - 5
ER -