Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity

Y. J. Tsai, H. Abe, H. Maruta, T. Hatano, H. Nishina, H. Sakagami, T. Okuda, S. I. Tanuma

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Abstract

Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.

Original languageEnglish
Pages (from-to)889-897
Number of pages9
JournalBiochemistry International
Volume24
Issue number5
Publication statusPublished - Dec 3 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Tsai, Y. J., Abe, H., Maruta, H., Hatano, T., Nishina, H., Sakagami, H., Okuda, T., & Tanuma, S. I. (1991). Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity. Biochemistry International, 24(5), 889-897.