Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity

Y. J. Tsai; H. Abe; H. Maruta; T. Hatano; H. Nishina; H. Sakagami; T. Okuda; S. I. Tanuma

Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity

Y. J. Tsai, H. Abe, H. Maruta, T. Hatano, H. Nishina, H. Sakagami, T. Okuda, S. I. Tanuma

Research output: Contribution to journal › Article › peer-review

Abstract

Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.

Original language	English
Pages (from-to)	889-897
Number of pages	9
Journal	Biochemistry International
Volume	24
Issue number	5
Publication status	Published - Dec 3 1991
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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title = "Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity",

abstract = "Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.",

author = "Tsai, {Y. J.} and H. Abe and H. Maruta and T. Hatano and H. Nishina and H. Sakagami and T. Okuda and Tanuma, {S. I.}",

year = "1991",

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T1 - Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity

AU - Tsai, Y. J.

AU - Abe, H.

AU - Maruta, H.

AU - Hatano, T.

AU - Nishina, H.

AU - Sakagami, H.

AU - Okuda, T.

AU - Tanuma, S. I.

PY - 1991/12/3

Y1 - 1991/12/3

N2 - Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.

AB - Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.

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Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity

Abstract

ASJC Scopus subject areas

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