Abstract
Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.
| Original language | English |
|---|---|
| Pages (from-to) | 889-897 |
| Number of pages | 9 |
| Journal | Biochemistry International |
| Volume | 24 |
| Issue number | 5 |
| Publication status | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity. / Tsai, Y. J.; Abe, H.; Maruta, H.; Hatano, Tsutomu; Nishina, H.; Sakagami, H.; Okuda, T.; Tanuma, S. I.
In: Biochemistry International, Vol. 24, No. 5, 1991, p. 889-897.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Effects of chemically defined tannins on Poly(ADP-ribose) glycohydrolase activity
AU - Tsai, Y. J.
AU - Abe, H.
AU - Maruta, H.
AU - Hatano, Tsutomu
AU - Nishina, H.
AU - Sakagami, H.
AU - Okuda, T.
AU - Tanuma, S. I.
PY - 1991
Y1 - 1991
N2 - Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.
AB - Three classes of chemically defined tannins, gallotannins, ellagitannins and condensed tannins were examined for their inhibitory activities against purified poly(ADP-ribose) glycohydrolase. Ellagitannins showed higher inhibitory activities than gallotannins. In contrant, condensed tannins, which consist of an epicathechin gallate (ECG) oligomer without a glucose core were not appreciably inhibitory. Kinetic analysis revealed that the inhibition of ellagitannins was competitive with respect to the substrate poly(ADP-ribose), whereas gallotannins exhibited mixed-type inhibition. These results suggest that conjugation with glucose of hexahydroxydiphenoyl (HHDP) group, which is a unique component of ellagitannins, potentiated the inhibitory activity, and that the structure of ellagitannins may have a functional domain which competes with poly(ADP-ribose) on the poly(ADP-ribose) glycohydrolase molecule.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025939794&partnerID=8YFLogxK
M3 - Article
C2 - 1776956
AN - SCOPUS:0025939794
VL - 24
SP - 889
EP - 897
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 5
ER -