Effects of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.)

M. Murata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In order to know the mutagenic effects of synthetic auxins (NAA, 2,4-D, and 2,4,5-T) and a cytokinin (kinetin) in vitro, sister chromatid exchanges (SCEs) were analyzed in cultured cells of a hexaploid wheat (Triticum aestivum L.). In the MS medium supplemented with 2.0 mg/l 2,4-D, the mean number of SCEs per cell was 15.2, and per pg of DNA, 0.42. No significant effect was found in the treatments of NAA or 2,4-D at concentrations of 0.5-10.0 mg/l, whereas more than 2.0 mg/l of 2,4,5-T induced dramatic increases of SCEs. Kinetin itself had no significant effect on SCE induction, but there was a tendency that SCEs induced by 2,4,5-T were suppressed by kinetin.

Original languageEnglish
Pages (from-to)521-524
Number of pages4
JournalTheoretical And Applied Genetics
Volume78
Issue number4
DOIs
Publication statusPublished - Oct 1989
Externally publishedYes

Fingerprint

Cytokinins
Sister Chromatid Exchange
Indoleacetic Acids
crossing over
cultured cells
cytokinins
2,4,5-Trichlorophenoxyacetic Acid
Triticum
auxins
Cultured Cells
2,4,5-T
Kinetin
Triticum aestivum
2,4-Dichlorophenoxyacetic Acid
wheat
kinetin
2,4-D
naphthaleneacetic acid
hexaploidy
DNA

Keywords

  • Auxin
  • Cytokinin
  • SCEs
  • Tissue culture
  • Triticum aestivum

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Effects of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.). / Murata, M.

In: Theoretical And Applied Genetics, Vol. 78, No. 4, 10.1989, p. 521-524.

Research output: Contribution to journalArticle

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