Effects of antidepressants on γ-aminobutyric acid- and N-methyl-D- aspartate-induced intracellular Ca2+ concentration increases in primary cultured rat cortical neurons

Minoru Takebayashi, Ariyuki Kagaya, Masatoshi Inagaki, Tosiro Kozuru, Hiroaki Jitsuiki, Kenichi Kurata, Yasumasa Okamoto, Shigeto Yamawaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We investigated the effects of antidepressants on the intracellular Ca2+ concentration ([Ca2+](i)) increases induced by γ-aminobutyric acid (GABA) or N-methyl-D-aspartate (NMDA) in primary cultured rat cortical neurons using fluorescence imaging. Acute treatment with imipramine inhibited GABA- and NMDA-induced increases in [Ca2+](i) in a concentration-dependent manner. Doses of 30 μM clomipramine, desipramine, amoxapine and maprotiline also inhibited both the GABA- and NMDA-induced [Ca2+](i) increases significantly. Both inhibitory effects of the five major antidepressants on the GABA- or the NMDA-induced [Ca2+](i) increases were well-correlated. Imipramine could inhibit significantly high- K+-induced [Ca2+](i) increases. Our previous study has already shown that the GABA-induced [Ca2+](i) increase involves a similar pathway to high-K+-induced Ca2+ influx. In conclusion, imipramine and several other antidepressants have acute inhibitory effects on the GABA-, NMDA- and high-K+-induced [Ca2+](i) increases, suggesting that these inhibitory effects are not related to specific receptors. One possibility is that these effects may be commonly mediated via part of the high-K+-induced [Ca2+](i) pathway. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)120-126
Number of pages7
JournalNeuropsychobiology
Volume42
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Antidepressant
  • Cortical neuron
  • Intracellular Ca
  • N-methyl-D-aspartate
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Psychiatry and Mental health
  • Biological Psychiatry

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