Abstract
We investigated the effects of antidepressants on the intracellular Ca2+ concentration ([Ca2+](i)) increases induced by γ-aminobutyric acid (GABA) or N-methyl-D-aspartate (NMDA) in primary cultured rat cortical neurons using fluorescence imaging. Acute treatment with imipramine inhibited GABA- and NMDA-induced increases in [Ca2+](i) in a concentration-dependent manner. Doses of 30 μM clomipramine, desipramine, amoxapine and maprotiline also inhibited both the GABA- and NMDA-induced [Ca2+](i) increases significantly. Both inhibitory effects of the five major antidepressants on the GABA- or the NMDA-induced [Ca2+](i) increases were well-correlated. Imipramine could inhibit significantly high- K+-induced [Ca2+](i) increases. Our previous study has already shown that the GABA-induced [Ca2+](i) increase involves a similar pathway to high-K+-induced Ca2+ influx. In conclusion, imipramine and several other antidepressants have acute inhibitory effects on the GABA-, NMDA- and high-K+-induced [Ca2+](i) increases, suggesting that these inhibitory effects are not related to specific receptors. One possibility is that these effects may be commonly mediated via part of the high-K+-induced [Ca2+](i) pathway. Copyright (C) 2000 S. Karger AG, Basel.
Original language | English |
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Pages (from-to) | 120-126 |
Number of pages | 7 |
Journal | Neuropsychobiology |
Volume | 42 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jan 1 2000 |
Keywords
- Antidepressant
- Cortical neuron
- Intracellular Ca
- N-methyl-D-aspartate
- γ-Aminobutyric acid
ASJC Scopus subject areas
- Neuropsychology and Physiological Psychology
- Psychiatry and Mental health
- Biological Psychiatry