Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats

Ichiro Yuasa, Kazunori Tsukuda, Ryuji Hirai, Tetsuya Ota, Masakazu Murakami, Minoru Naito, Hiroyoshi Doihara, Hiroshi Date, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Resection is the only curative treatment for liver metastasis of colorectal cancers. Despite the supreme regenerative potential of the liver, major hepatectomy sometimes leads to liver failure, and the limitation of resectable liver volumes makes advanced tumors inoperable. This study was attempted to promote liver regeneration using hepatocyte growth factor (HGF) gene transfection by venous-administered adenovirus and to improve the survival of rats after massive hepatectomy. The adenovirus that encodes HGF was administered to rats before 85%-hepatectomy. The administration of HGF gene improved the survival of rats after massive hepatectomy, while the administration of control adenovirus deteriorated their survival. Gene transfection of HGF showed up-regulation of serum HGF, stimulation of hepatocellular proliferation and rapid liver regeneration. Moreover, HGF administration reduced apoptosis of hepatocytes. The administration of HGF gene prevented liver dysfunction after major hepatectomy and may be a new assist for surgery. Copyright

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalActa Medica Okayama
Volume61
Issue number2
Publication statusPublished - Apr 2007

Fingerprint

Liver Regeneration
Hepatocyte Growth Factor
Hepatectomy
Liver
Rats
Genes
Adenoviridae
Transfection
Liver Failure
Surgery
Liver Diseases
Tumors
Hepatocytes
Colorectal Neoplasms
Up-Regulation
Apoptosis
Neoplasm Metastasis
Serum

Keywords

  • Adenoviral vector
  • Gene therapy
  • Hepatectomy
  • HGF

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Yuasa, I., Tsukuda, K., Hirai, R., Ota, T., Murakami, M., Naito, M., ... Shimizu, N. (2007). Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats. Acta Medica Okayama, 61(2), 81-88.

Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats. / Yuasa, Ichiro; Tsukuda, Kazunori; Hirai, Ryuji; Ota, Tetsuya; Murakami, Masakazu; Naito, Minoru; Doihara, Hiroyoshi; Date, Hiroshi; Shimizu, Nobuyoshi.

In: Acta Medica Okayama, Vol. 61, No. 2, 04.2007, p. 81-88.

Research output: Contribution to journalArticle

Yuasa, I, Tsukuda, K, Hirai, R, Ota, T, Murakami, M, Naito, M, Doihara, H, Date, H & Shimizu, N 2007, 'Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats', Acta Medica Okayama, vol. 61, no. 2, pp. 81-88.
Yuasa I, Tsukuda K, Hirai R, Ota T, Murakami M, Naito M et al. Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats. Acta Medica Okayama. 2007 Apr;61(2):81-88.
Yuasa, Ichiro ; Tsukuda, Kazunori ; Hirai, Ryuji ; Ota, Tetsuya ; Murakami, Masakazu ; Naito, Minoru ; Doihara, Hiroyoshi ; Date, Hiroshi ; Shimizu, Nobuyoshi. / Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats. In: Acta Medica Okayama. 2007 ; Vol. 61, No. 2. pp. 81-88.
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