Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats

Ichiro Yuasa, Kazunori Tsukuda, Ryuji Hirai, Tetsuya Ota, Masakazu Murakami, Minoru Naito, Hiroyoshi Doihara, Hiroshi Date, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Resection is the only curative treatment for liver metastasis of colorectal cancers. Despite the supreme regenerative potential of the liver, major hepatectomy sometimes leads to liver failure, and the limitation of resectable liver volumes makes advanced tumors inoperable. This study was attempted to promote liver regeneration using hepatocyte growth factor (HGF) gene transfection by venous-administered adenovirus and to improve the survival of rats after massive hepatectomy. The adenovirus that encodes HGF was administered to rats before 85%-hepatectomy. The administration of HGF gene improved the survival of rats after massive hepatectomy, while the administration of control adenovirus deteriorated their survival. Gene transfection of HGF showed up-regulation of serum HGF, stimulation of hepatocellular proliferation and rapid liver regeneration. Moreover, HGF administration reduced apoptosis of hepatocytes. The administration of HGF gene prevented liver dysfunction after major hepatectomy and may be a new assist for surgery. Copyright

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalActa medica Okayama
Volume61
Issue number2
Publication statusPublished - Apr 2007

Keywords

  • Adenoviral vector
  • Gene therapy
  • HGF
  • Hepatectomy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Yuasa, I., Tsukuda, K., Hirai, R., Ota, T., Murakami, M., Naito, M., Doihara, H., Date, H., & Shimizu, N. (2007). Effects of adenoviral-mediated hepatocyte growth factor on liver regeneration after massive hepatectomy in rats. Acta medica Okayama, 61(2), 81-88.