Effects of active silicon uptake by rice on 29Si fractionation in various plant parts

Jan Reent Köster, Roland Bol, Melanie J. Leng, Adrian G. Parker, Hilary J. Sloane, Jian Feng Ma

Research output: Contribution to journalArticle

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Abstract

Rice (Oryza sativa L.) accumulates large amounts of silicon which improves its growth and health due to enhanced resistance to biotic and abiotic stresses. Silicon uptake and loading to xylem in rice are predominantly active processes performed by transporters encoded by the recently identified genes Lsil (Si influx transporter gene) and Lsi2 (Si efflux transporter gene). Silicon deposition in rice during translocation to upper plant tissues is known to discriminate against the heavier isotopes 29Si and 30Si, resulting in isotope fractionation within the plant. We analyzed straw and husk samples of rice mutants defective in Lsi1, Lsi2 or both for silicon content and δ29Si using isotope ratio mass spectrometry (IRMS) and compared these results with those for the corresponding wild-type varieties (WT). The silicon content was higher in husk than in straw. All the mutant rice lines showed clearly lower silicon content than the WT lines (4-23% Si of WT). The δ29Si was lower in straw and husk for the uptake defective mutant (lsi1) than for WT, albeit δ29Si was 0.3‰ higher in husk than in straw in both lines. The effect of defective efflux (lsi2) differed for straw and husk with higher δ29Si in straw, but lower δ29Si in husk while WT showed similar δ29Si in both fractions. These initial results show the potential of Si isotopes to enlighten the influence of active uptake on translocation and deposition processes in the plant.

Original languageEnglish
Pages (from-to)2398-2402
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume23
Issue number16
DOIs
Publication statusPublished - Aug 30 2009

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Straw
Silicon
Fractionation
Isotopes
Genes
Mass spectrometry
Health
Tissue

ASJC Scopus subject areas

  • Spectroscopy
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Effects of active silicon uptake by rice on 29Si fractionation in various plant parts. / Köster, Jan Reent; Bol, Roland; Leng, Melanie J.; Parker, Adrian G.; Sloane, Hilary J.; Ma, Jian Feng.

In: Rapid Communications in Mass Spectrometry, Vol. 23, No. 16, 30.08.2009, p. 2398-2402.

Research output: Contribution to journalArticle

Köster, Jan Reent ; Bol, Roland ; Leng, Melanie J. ; Parker, Adrian G. ; Sloane, Hilary J. ; Ma, Jian Feng. / Effects of active silicon uptake by rice on 29Si fractionation in various plant parts. In: Rapid Communications in Mass Spectrometry. 2009 ; Vol. 23, No. 16. pp. 2398-2402.
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