Effects of (+)-8',8',8'-trifluoroabscisic acid on α-amylase expression and sugar accumulation in rice cells

M. A. Kashem, H. Hori, K. Itoh, T. Hayakawa, Y. Todoroki, N. Hirai, H. Ohigashi, T. Mitsui

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The effects of (+)-8',8',8'-trifluoroabscisic acid (trifluoro-ABA) on α-amylase expression were studied in rice embryoless half-seeds, scutella, and suspension-cultured cells derived from the embryo, and the effects of the analog on sugar accumulation were also studied in scutella and suspension-cultured cells. Treatment with (+)-trifluoro-ABA strongly inhibited the gibberellic acid-inducible expression of α-amylase I-1 encoded by RAmy1A in the aleurone layers of embryoless half-seeds at the levels of transcription, protein synthesis, and enzyme activity. It was also found that (+)-trifluoro-ABA stimulated (i) the uptake of glucose from the incubation medium and (ii) the synthesis of sucrose in scutellar tissues and suspension-cultured cells of rice. The biological activity of (+)-trifluoro-ABA was found to be more potent and persistent than that of natural ABA. We further examined the effects of trifluoro-ABA on the expression of α-amylase I-1 in scutellar tissues and suspension-cultured cells. It was found that (+)-trifluoro-ABA did not inhibit the formation of α-amylase I-1 in the absence of external glucose. However, glucose and (+)-trifluoro-ABA cooperatively suppressed the formation of α-amylase I-1. Judging from these results, we conclude that the regulatory mechanism for the expression of α-amylase I-1 in the scutellar epithelium is distinguishable from that operating in the aleurone layer.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalPlanta
Volume205
Issue number3
DOIs
Publication statusPublished - Jul 1998
Externally publishedYes

Keywords

  • Abscisic acid (analog)
  • Gene expression (RAmylA)
  • Oryza (α-amylase, sugar accumulation)
  • Sugar accumulation (rice)
  • α-Amylase I-1

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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