Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of the OsHMA2 promoter

Shao Jifeng, Jixing Xia, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Reducing cadmium (Cd) accumulation in rice grain is an important issue for human health. The aim of this study was to manipulate both expression and tissue localization of OsHMA3, a tonoplast-localized Cd transporter, in the roots by expressing it under the control of the OsHMA2 promoter, which shows high expression in different organs including roots, nodes, and shoots. In two independent transgenic lines, the expression of OsHMA3 was significantly enhanced in all organs compared with non-transgenic rice. Furthermore, OsHMA3 protein was detected in the root pericycle cells and phloem region of both the diffuse vascular bundle and the enlarged vascular bundle of the nodes. At the vegetative stage, the Cd concentration in the shoots and xylem sap of the transgenic rice was significantly decreased, but that of the whole roots and root cell sap was increased. At the reproductive stage, the concentration of Cd, but not other essential metals, in the brown rice of transgenic lines was decreased to less than one-tenth that of the non-transgenic rice. These results indicate that expression of OsHMA3 under the control of the OsHMA2 promoter can effectively reduce Cd accumulation in rice grain through sequestering more Cd into the vacuoles of various tissues.

Original languageEnglish
Pages (from-to)2743-2752
Number of pages10
JournalJournal of Experimental Botany
Volume69
Issue number10
DOIs
Publication statusPublished - Apr 27 2018

Fingerprint

Cadmium
cadmium
promoter regions
rice
vascular bundles
genetically modified organisms
sap
Blood Vessels
Phloem
Xylem
shoots
brown rice
tonoplast
Vacuoles
phloem
vegetative growth
vacuoles
xylem
transporters
human health

Keywords

  • Cadmium
  • node
  • OsHMA2
  • OsHMA3
  • transgenic rice
  • vacuolar sequestration

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Effective reduction of cadmium accumulation in rice grain by expressing OsHMA3 under the control of the OsHMA2 promoter. / Jifeng, Shao; Xia, Jixing; Yamaji, Naoki; Shen, Ren Fang; Ma, Jian Feng.

In: Journal of Experimental Botany, Vol. 69, No. 10, 27.04.2018, p. 2743-2752.

Research output: Contribution to journalArticle

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