TY - JOUR
T1 - Effective pumping proton collection facilitated by a copper site (Cu B) of bovine heart cytochrome c oxidase, revealed by a newly developed time-resolved infrared system
AU - Kubo, Minoru
AU - Nakashima, Satoru
AU - Yamaguchi, Satoru
AU - Ogura, Takashi
AU - Mochizuki, Masao
AU - Kang, Jiyoung
AU - Tateno, Masaru
AU - Shinzawa-Itoh, Kyoko
AU - Kato, Koji
AU - Yoshikawa, Shinya
PY - 2013/10/18
Y1 - 2013/10/18
N2 - Background: Cytochrome c oxidase reduces O2 coupled with proton pumping. Results: A newly developed time-resolved infrared system reveals transient conformational changes in the proton-pumping pathway upon CO binding to CuB in the O2 reduction site. Conclusion: Cu B promotes proton collection and effective blockage of back-leak of pumping protons. Significance: These critical findings in bioenergetics stimulate the new infrared approach for mechanistic investigation of any other protein function.
AB - Background: Cytochrome c oxidase reduces O2 coupled with proton pumping. Results: A newly developed time-resolved infrared system reveals transient conformational changes in the proton-pumping pathway upon CO binding to CuB in the O2 reduction site. Conclusion: Cu B promotes proton collection and effective blockage of back-leak of pumping protons. Significance: These critical findings in bioenergetics stimulate the new infrared approach for mechanistic investigation of any other protein function.
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U2 - 10.1074/jbc.M113.473983
DO - 10.1074/jbc.M113.473983
M3 - Article
C2 - 23996000
AN - SCOPUS:84886871840
SN - 0021-9258
VL - 288
SP - 30259
EP - 30269
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 42
ER -