Effective pumping proton collection facilitated by a copper site (Cu B) of bovine heart cytochrome c oxidase, revealed by a newly developed time-resolved infrared system

Minoru Kubo, Satoru Nakashima, Satoru Yamaguchi, Takashi Ogura, Masao Mochizuki, Jiyoung Kang, Masaru Tateno, Kyoko Shinzawa-Itoh, Koji Kato, Shinya Yoshikawa

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Background: Cytochrome c oxidase reduces O2 coupled with proton pumping. Results: A newly developed time-resolved infrared system reveals transient conformational changes in the proton-pumping pathway upon CO binding to CuB in the O2 reduction site. Conclusion: Cu B promotes proton collection and effective blockage of back-leak of pumping protons. Significance: These critical findings in bioenergetics stimulate the new infrared approach for mechanistic investigation of any other protein function.

Original languageEnglish
Pages (from-to)30259-30269
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
Publication statusPublished - Oct 18 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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