TY - JOUR
T1 - Effective production of dehydro cyclic dipeptide albonoursin exhibiting pronuclear fusion inhibitory activity. I. Taxonomy and fermentation
AU - Kanzaki, Hiroshi
AU - Imura, Daisuke
AU - Sashida, Reiko
AU - Kobayashi, Akio
AU - Kawazu, Kazuyoshi
PY - 1999/11
Y1 - 1999/11
N2 - Strain KO-23, an actinomycete producing albonoursin as well as streptopyrone, was identified as Streptomyces albulus by morphological and biochemical studies. Fermentation conditions for albonoursin, a dehydro cyclic dipeptide exhibiting a pronounced inhibitory activity toward pronuclear fusion of sea urchin eggs, were optimized. Under the optimum conditions, the actinomycete produced 16 mg/liter of albonoursin, 30 times higher than that in the original culture. The cells cultivated under these conditions highly express biosynthetic enzymes for albonoursin, and thus are available for biosynthetic studies of dehydro cyclic peptides.
AB - Strain KO-23, an actinomycete producing albonoursin as well as streptopyrone, was identified as Streptomyces albulus by morphological and biochemical studies. Fermentation conditions for albonoursin, a dehydro cyclic dipeptide exhibiting a pronounced inhibitory activity toward pronuclear fusion of sea urchin eggs, were optimized. Under the optimum conditions, the actinomycete produced 16 mg/liter of albonoursin, 30 times higher than that in the original culture. The cells cultivated under these conditions highly express biosynthetic enzymes for albonoursin, and thus are available for biosynthetic studies of dehydro cyclic peptides.
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U2 - 10.7164/antibiotics.52.1017
DO - 10.7164/antibiotics.52.1017
M3 - Article
C2 - 10656574
VL - 52
SP - 1017
EP - 1022
JO - Journal of Antibiotics
JF - Journal of Antibiotics
SN - 0021-8820
IS - 11
ER -