Effect of Yersinia enterocolitica ST on Cyclic Guanosine 3′, 5′‐Monophosphate Levels in Mouse Intestines and Cultured Cells

Takashi Inoue, Keinosuke Okamoto, Takeshi Moriyama, Tetsuomi Takahashi, Kiyoji Shimizu, Akio Miyama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We determined the effect of heat‐stable enterotoxin produced by Yersinia enterocolitica (Y. enterocolitica ST) on cyclic nucleotide levels in the intestines of 6‐day‐old mice and in cultured cell line cells. The concentration of cyclic guanosine 3′,5′‐monophosphate (cyclic GMP) in homogenates of the intestines increased four‐ to fivefold by 3 min after intragastric administration of 10 units of purified Y. enterocolitica ST. This increase continued for 60 min, and then the concentration of cyclic GMP fell toward the levels of the controls. On the other hand, fluid accumulation in the intestines was not evident until 60 min after administration of the toxin. Thus, the increase in intestinal cyclic GMP concentration preceded measurable fluid accumulation. The effect on both cyclic GMP levels and fluid accumulation was abolished by treatment of the ST with either alkali solution (pH 10.7) or 2‐mercaptoethanol. Likewise, cyclic GMP levels in cultured cells (CCL‐6, HeLa, L, and Mm‐1 cells) increased dose‐dependently by 10 min after incubation of the cells with the ST. Cyclic adenosine 3′,5′‐monophosphate levels in both intestines and cultured cells were not affected by the toxin.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalMicrobiology and Immunology
Volume27
Issue number2
DOIs
Publication statusPublished - 1983
Externally publishedYes

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Yersinia enterocolitica
Guanosine
Intestines
Cultured Cells
Enterotoxins
Cyclic Nucleotides
Alkalies
Adenosine
Cell Line

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Effect of Yersinia enterocolitica ST on Cyclic Guanosine 3′, 5′‐Monophosphate Levels in Mouse Intestines and Cultured Cells. / Inoue, Takashi; Okamoto, Keinosuke; Moriyama, Takeshi; Takahashi, Tetsuomi; Shimizu, Kiyoji; Miyama, Akio.

In: Microbiology and Immunology, Vol. 27, No. 2, 1983, p. 159-166.

Research output: Contribution to journalArticle

Inoue, Takashi ; Okamoto, Keinosuke ; Moriyama, Takeshi ; Takahashi, Tetsuomi ; Shimizu, Kiyoji ; Miyama, Akio. / Effect of Yersinia enterocolitica ST on Cyclic Guanosine 3′, 5′‐Monophosphate Levels in Mouse Intestines and Cultured Cells. In: Microbiology and Immunology. 1983 ; Vol. 27, No. 2. pp. 159-166.
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