Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells

Koichi Shibuya; Shoji Kawasaki; Masahiro Kuroda; Mitsuhiro Takemoto; Jun Ichi Asaumi; Yoshio Hiraki

doi:10.3892/or.9.1.81

Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells

Koichi Shibuya, Shoji Kawasaki, Masahiro Kuroda, Mitsuhiro Takemoto, Jun Ichi Asaumi, Yoshio Hiraki

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 μM, but not dose-dependent from 0.1 to 20 μM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	Oncology reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.3892/or.9.1.81
Publication status	Published - Jan 2002

Keywords

PI-3 kinase
PKC
Thermotolerance
TsAF8
Wortmannin

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3892/or.9.1.81

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abstract = "Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 μM, but not dose-dependent from 0.1 to 20 μM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.",

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AU - Shibuya, Koichi

AU - Kawasaki, Shoji

AU - Kuroda, Masahiro

AU - Takemoto, Mitsuhiro

AU - Asaumi, Jun Ichi

AU - Hiraki, Yoshio

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AB - Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 μM, but not dose-dependent from 0.1 to 20 μM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.

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Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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