Effect of water deprivation on aquaporin 4 (AQP4) mRNA expression in chickens (Gallus domesticus)

Noboru Saito, Hidehiro Ikegami, Kiyoshi Shimada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aquaporin (AQP) 4 is a member of the AQP gene family of water-selective transport proteins. We studied the effect of water deprivation on AQP4 gene expression in chickens. The nucleotide sequence of a chicken aquaporin 4 (AQP4) cDNA that encodes a protein of 335 amino acids showed high homology to mammalian AQP4. Using Northern blotting analysis, AQP4 mRNA in chickens was observed as a band of approximately 5.5 kb in several tissues in addition to the hypothalamus, proventriculus, kidney, and breast muscle. Quantitative analysis by real-time RT-PCR analysis showed that the mRNA expression of AQP4 in the hypothalamus significantly increased after dehydration. On the other hand, the mRNA expression of AQP4 in the kidney significantly decreased after dehydration. This suggests that AQP4 may play a pivotal role in osmoregulation in the chicken brain.

Original languageEnglish
Pages (from-to)193-197
Number of pages5
JournalMolecular Brain Research
Volume141
Issue number2
DOIs
Publication statusPublished - Nov 30 2005
Externally publishedYes

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Keywords

  • Aquaporin 4 (AQP4)
  • cDNA cloning
  • Chicken
  • Gene expression
  • Real-time PCR
  • Water deprivation

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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